多瘤病毒感染3T3小鼠成纤维细胞诱导的DNA聚合酶活性
DNA polymerase activities induced by polyoma virus infection of 3T3 mouse fibroblasts.
作者信息
Närkhammar M, Magnusson G
出版信息
J Virol. 1976 Apr;18(1):1-6. doi: 10.1128/JVI.18.1.1-6.1976.
Abstract
Resting mouse 3T3 fibroblasts were stimulated to synthesize DNA either by infection with polyoma virus or by injection of fresh serum. Changes in the levels of DNA polymerase (alpha-, beta-, and gamma-enzymes) were measured in the cytoplasm and the cell nucleus. Both types of stimulation gave very similar increases for all three enzyme activities. In the cell nucleus, both alpha- and gamma-polymerases increased almost tenfold, whereas the beta-enzyme only was stimulated twofold. In the cytoplasm alpha- and gamma-polymerases increased two- to four-fold. Only insignificant amounts of the beta-enzyme were found in the cytoplasm.
摘要
静止的小鼠3T3成纤维细胞通过感染多瘤病毒或注射新鲜血清来刺激其合成DNA。在细胞质和细胞核中测量了DNA聚合酶（α、β和γ酶）水平的变化。两种刺激类型对所有三种酶活性都产生了非常相似的增加。在细胞核中，α和γ聚合酶几乎增加了十倍，而β酶仅被刺激了两倍。在细胞质中，α和γ聚合酶增加了两到四倍。在细胞质中仅发现少量的β酶。
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