Sciortino Sonia, Arculeo Pietro, Alio Vincenzina, Cardamone Cinzia, Nicastro Luisa, Arculeo Marco, Alduina Rosa, Costa Antonella
Food Microbiology Section, Istituto Zooprofilattico Sperimentale della Sicilia A. Mirri, Via G. Marinuzzi 3, 90129 Palermo, Italy.
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, Bd. 16, 90128 Palermo, Italy.
Antibiotics (Basel). 2021 Mar 10;10(3):288. doi: 10.3390/antibiotics10030288.
spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of isolates recovered from different aquatic sources. Besides, we searched for spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of than the corresponding seawater samples. The isolates were identified as (96.4%) and (3.6%). All the isolates showed a multi-drug resistance profile, being resistant to at least three different classes of antibiotics. Molecular analysis of genetic determinants responsible for tetracycline resistance in nine randomly chosen isolates revealed the presence of and/or This work confirms the occurrence and the continuous emergence of antibiotic-resistant strains in environmental samples; also, the presence of quinolone-resistant spp. in aquatic sources used for water supply and irrigation represents a potential risk for human health.
某些物种是新出现的水源性和食源性人畜共患病原体，可导致人类肠胃炎。在这项工作中，我们评估了从不同水源分离出的菌株的发生率和抗菌药物耐药性概况。此外，我们在海藻和相应的海水样本中寻找某些物种。应用于100个样本的细菌学和分子方法从27个样本中分离出28株菌株。在河流中检测到的患病率最高，其次是人工池塘、溪流、井水和泉水。海藻中所含某些物种的比例高于相应的海水样本。分离出的菌株被鉴定为某物种（96.4%）和另一物种（3.6%）。所有分离菌株均呈现多重耐药性，对至少三类不同抗生素具有抗性。对九个随机选择的分离菌株中负责四环素抗性的遗传决定因素进行分子分析，发现存在某基因和/或另一基因。这项工作证实了环境样本中抗生素耐药性某菌株的存在和持续出现；此外，用于供水和灌溉的水源中存在喹诺酮耐药性某物种对人类健康构成潜在风险。
