Lindén L, Källberg M, Gustafson A, Dahlén G
Scand J Clin Lab Invest. 1976 Jan;36(1):51-8. doi: 10.1080/00365517609068018.
The occurrence on cellulose acetate and agarose gel electrophoresis of additional bands with the mobility of pre-beta-lipoprotein (LP) has recently been linked to ischemic heart disease (IHD). In the present study, one of these additional pre-beta-LP fractions, earlier designated as 'pre-beta-LP' and now defined on agarose gel electrophoresis, was isolated by preparative ultracentrifugation and gel filtration. The characterization of this pre-beta-1-LP revealed a 'sinking pre-beta-LP' (SPB) of density 1.050-1.080 g/ml. SPB shared its major antigenic determinant with low-density lipoproteins (LDL) and resembled closely LDL with regard to its lipid composition. Specific anti-LP(a) serum confirmed the presence of LP(a) antigen in the purified SPB preparation.
最近，在醋酸纤维素和琼脂糖凝胶电泳上出现的具有前β-脂蛋白（LP）迁移率的额外条带与缺血性心脏病（IHD）有关。在本研究中，通过制备性超速离心和凝胶过滤分离出了这些额外的前β-LP组分之一，该组分先前被指定为“前β-LP”，现在通过琼脂糖凝胶电泳进行定义。对这种前β-1-LP的表征显示其为密度为1.050 - 1.080 g/ml的“下沉前β-LP”（SPB）。SPB与低密度脂蛋白（LDL）共享其主要抗原决定簇，并且在脂质组成方面与LDL非常相似。特异性抗LP（a）血清证实了纯化的SPB制剂中存在LP（a）抗原。
