Young Chiu Chung, Ho Mann-Jing, Arun A B, Chen Wen-Ming, Lai Wei-An, Shen Fo-Ting, Rekha P D, Yassin A F
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China.
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Institute of Marine Technology, Nan-Tzu, Kaohsiung 811, Taiwan, Republic of China.
Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1823-1827. doi: 10.1099/ijs.0.65053-0.
A bacterial isolate from a sample of oil-contaminated soil was characterized using a polyphasic taxonomic approach. Comparative analysis of the 16S rRNA gene sequence showed that this isolate constituted a distinct phyletic line within the genus Pseudoxanthomonas, displaying >3.7 % sequence divergence with respect to recognised Pseudoxanthomonas species. The genus assignment was confirmed by a chemotaxonomic analysis, which revealed the presence of a fatty acid profile characteristic of members of the genus Pseudoxanthomonas (straight-chain saturated, unsaturated and branched-chain fatty acids of the iso/anteiso type and 3-hydroxylated fatty acids) and the presence of a ubiquinone with eight isoprene units (Q-8) as the predominant respiratory quinone. The novel isolate was distinguishable from other members of the genus Pseudoxanthomonas on the basis of a combination of phenotypic properties. The genotypic and phenotypic data show that the strain represents a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas spadix sp. nov. is proposed. The type strain is IMMIB AFH-5(T) (=DSM 18855(T)=CCUG 53828(T)).
采用多相分类学方法对从受油污染土壤样本中分离出的一株细菌进行了鉴定。16S rRNA基因序列的比较分析表明，该分离株在假黄单胞菌属内构成了一个独特的进化系，与已确认的假黄单胞菌属物种相比，序列差异大于3.7%。化学分类学分析证实了该属的归属，该分析揭示了具有假黄单胞菌属成员特征的脂肪酸谱（直链饱和、不饱和和异/anteiso型支链脂肪酸以及3-羟基脂肪酸）的存在，以及以具有八个异戊二烯单元的泛醌（Q-8）作为主要呼吸醌的存在。基于表型特性的组合，该新分离株与假黄单胞菌属的其他成员有所区别。基因型和表型数据表明，该菌株代表假黄单胞菌属的一个新物种，为此提出名称为Pseudoxanthomonas spadix sp. nov.。模式菌株为IMMIB AFH-5(T)（=DSM 18855(T)=CCUG 53828(T)）。
