Resende Flávia Aparecida, Alves Jacqueline Morais, Munari Carla Carolina, Senedese Juliana Marques, Sousa João Paulo B, Bastos Jairo Kenupp, Tavares Denise Crispim
Universidade de Franca, Avenida Dr Armando Salles de Oliveira, 201-Parque Universitário, 14404-600 Franca, São Paulo, Brazil.
Mutat Res. 2007 Dec 1;634(1-2):112-8. doi: 10.1016/j.mrgentox.2007.06.008. Epub 2007 Jun 30.
Baccharis dracunculifolia DC (Asteraceae), a native plant from Brazil, have been used as an antipyretic, stomachic and health tonic in Brazil. The objective of the present study was to investigate the potential mutagenic effect of B. dracunculifolia ethyl acetate extract (Bd-EAE) and its influence on the mutagenicity induced by the chemotherapeutic agent doxorubicin (DXR) using the rat bone marrow and peripheral blood micronucleus test. Wistar rats were divided into 10 treatment groups. Five groups received DXR (90 mg/kg body weight, b.w., intraperitoneally) to induce mutagenicity and three of these groups received a single oral dose of Bd-EAE at a concentration of 6, 12 or 24 mg/kg b.w. prior to DXR administration. A vehicle-treated control group and Bd-EAE control groups were also included. The results showed that Bd-EAE itself was not mutagenic, in the rat micronucleus assay. In animals treated with Bd-EAE and DXR, the number of MNPCEs was significantly decreased compared to animals receiving DXR alone. HPLC analysis of the extract obtained permitted the identification of the following phenolic compounds: caffeic acid, p-coumaric acid, aromadendrin-4'O-methyl ether, 3-prenyl-p-coumaric acid (drupanin), 3,5-diprenyl-p-coumaric acid (artepillin C) and baccharin. The putative antioxidant activity or the interference of one or more of the active compounds of Bd-EAE with mutagenic metabolic pathways may explain its effect on DXR mutagenicity.
巴西腊菊（菊科）是一种原产于巴西的植物，在巴西被用作退烧药、健胃药和滋补品。本研究的目的是使用大鼠骨髓和外周血微核试验，研究巴西腊菊乙酸乙酯提取物（Bd-EAE）的潜在诱变作用及其对化疗药物阿霉素（DXR）诱导的诱变作用的影响。将Wistar大鼠分为10个治疗组。五组接受DXR（90mg/kg体重，腹腔注射）以诱导诱变，其中三组在给予DXR之前接受单剂量口服浓度为6、12或24mg/kg体重的Bd-EAE。还包括一个赋形剂处理的对照组和Bd-EAE对照组。结果表明，在大鼠微核试验中，Bd-EAE本身没有诱变作用。在用Bd-EAE和DXR处理的动物中，与单独接受DXR的动物相比，微核多染红细胞（MNPCEs）的数量显著减少。对所得提取物的HPLC分析允许鉴定以下酚类化合物：咖啡酸、对香豆酸、芳樟醇-4'O-甲基醚、3-异戊烯基-对香豆酸（drupanin）、3,5-二异戊烯基-对香豆酸（artepillin C）和baccharin。Bd-EAE的假定抗氧化活性或一种或多种活性化合物对诱变代谢途径的干扰可能解释了其对DXR诱变作用的影响。
