Cuesta-Rubio Osmany, Piccinelli Anna Lisa, Fernandez Mercedes Campo, Hernández Ingrid Márquez, Rosado Arístides, Rastrelli Luca
Instituto de Farmacia y Alimentos (IFAL), Universidad de La Habana, Ave. 23 No. 21425, Lisa, La Habana, Cuba.
J Agric Food Chem. 2007 Sep 5;55(18):7502-9. doi: 10.1021/jf071296w. Epub 2007 Aug 11.
Sixty-five samples of propolis were collected from eleven regions of Cuba; methanolic extracts of propolis were prepared from all samples, and a classification method was developed using a combination of NMR, HPLC-PDA, and HPLC-ESI/MS techniques. The analysis of (1)H and (13)C NMR spectra and chromatographic profiles of all propolis extracts allowed the definition of three main types of Cuban propolis directly related to their secondary metabolite classes: brown Cuban propolis (BCP), rich in polyisoprenylated benzophenones, red Cuban propolis (RCP), containing isoflavonoids as the main constituents, and yellow Cuban propolis (YCP), probably with aliphatic compounds. Subsequently, the principal compounds of the brown and red types were characterized by HPLC-ESI/MS analysis. Instrumental techniques used are complementary; NMR was shown to be a quick and informative tool for the rapid analysis of crude propolis polar extracts and allowed the identification of the main class of secondary metabolites, while LC-PDA and LC-MS techniques were useful tools for qualitative and quantitative analysis of marker compounds of Cuban propolis.
从古巴的11个地区收集了65个蜂胶样本；从所有样本中制备了蜂胶的甲醇提取物，并结合核磁共振（NMR）、高效液相色谱-光电二极管阵列检测（HPLC-PDA）和高效液相色谱-电喷雾电离/质谱（HPLC-ESI/MS）技术开发了一种分类方法。对所有蜂胶提取物的氢核磁共振（¹H NMR）和碳核磁共振（¹³C NMR）光谱以及色谱图进行分析，确定了古巴蜂胶的三种主要类型，这与它们的次生代谢物类别直接相关：富含多异戊烯基化二苯甲酮的棕色古巴蜂胶（BCP）、以异黄酮为主要成分的红色古巴蜂胶（RCP）以及可能含有脂肪族化合物的黄色古巴蜂胶（YCP）。随后通过HPLC-ESI/MS分析对棕色和红色类型的主要化合物进行了表征。所使用的仪器技术具有互补性；核磁共振被证明是快速分析粗制蜂胶极性提取物的一种快速且信息丰富的工具，能够鉴定次生代谢物的主要类别，而液相色谱-光电二极管阵列检测和液相色谱-质谱技术则是古巴蜂胶标记化合物定性和定量分析的有用工具。
