Matsuda Yasuhiro, Murata Yukie, Tanaka Toru, Komatsu Hisanori, Sano Hidehiko
Division of Oral Health Science, Department of Restorative Dentistry, Hokkaido University Graduate School of Dental Medicine, Kita 13, Nishi 7, Kita-ku, Sapporo, 060-8586, Japan.
Dent Mater J. 2007 May;26(3):414-21. doi: 10.4012/dmj.26.414.
To the end of developing a convenient research tool to calculate the mineralization status of teeth in detail, a new program was developed using Visual Basic for Applications combined with Microsoft Excel 2004. To demonstrate the usefulness of this program, it was used to analyze tooth enamel mineralization after acid exposure. Transverse microradiography images (TMR) of specimens were digitalized with a charge-coupled device camera with a microscope (CCD camera) and a digital film scanner (FS). Subsequently, the mineral content profile of each specimen after de- and remineralization studies were calculated using the Angmar's formula. The newly developed program was applied to calculating the mineral loss (DeltaZ), lesion depth (Ld), surface zone depth (SZd), and lesion body depth (LBd) of tooth specimens. In addition, the outer surface zone (OSZ), inner lesion body (ILB), and sandwich area (SA) between OSZ and ILB- which together constituted DeltaZ - were calculated by the newly developed program. Data obtained with the newly developed program were in good agreement for both CCD camera and FS, indicating that the program was reliable for tooth enamel mineralization research studies.
为了开发一种方便的研究工具来详细计算牙齿的矿化状态，使用应用程序可视化 Basic 结合 Microsoft Excel 2004 开发了一个新程序。为了证明该程序的实用性，将其用于分析酸暴露后牙釉质的矿化情况。用配备显微镜的电荷耦合器件相机（CCD 相机）和数字胶片扫描仪（FS）对标本的横向显微放射图像（TMR）进行数字化处理。随后，使用 Angmar 公式计算脱矿和再矿化研究后每个标本的矿物质含量分布。将新开发的程序应用于计算牙齿标本的矿物质损失（ΔZ）、病变深度（Ld）、表面区域深度（SZd）和病变体深度（LBd）。此外，新开发的程序计算了外表面区域（OSZ）、内部病变体（ILB）以及 OSZ 和 ILB 之间的夹层区域（SA），它们共同构成了 ΔZ。使用新开发的程序获得的数据在 CCD 相机和 FS 方面都具有良好的一致性，表明该程序对于牙釉质矿化研究是可靠的。
