Marjanović Damir, Durmić-Pasić Adaleta, Bakal Narcisa, Haverić Sanin, Kalamujić Belma, Kovacević Lejla, Ramić Jasmin, Pojskić Naris, Skaro Vedrana, Projić Petar, Bajrović Kasim, Hadziselimović Rifat, Drobnic Katja, Huffine Edwin, Davoren Jon, Primorac Dragan
Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina.
Croat Med J. 2007 Aug;48(4):513-9.
To present the joint effort of three institutions in the identification of human remains from the World War II found in two mass graves in the area of Skofja Loka, Slovenia.
The remains of 27 individuals were found in two small and closely located mass graves. The DNA was isolated from bone and teeth samples using either standard phenol/chloroform alcohol extraction or optimized Qiagen DNA extraction procedure. Some recovered samples required the employment of additional DNA purification methods, such as N-buthanol treatment. Quantifiler Human DNA Quantification Kit was used for DNA quantification. PowerPlex 16 kit was used to simultaneously amplify 15 short tandem repeat (STR) loci. Matching probabilities were estimated using the DNA View program.
Out of all processed samples, 15 remains were fully profiled at all 15 STR loci. The other 12 profiles were partial. The least successful profile included 13 loci. Also, 69 referent samples (buccal swabs) from potential living relatives were collected and profiled. Comparison of victims' profile against referent samples database resulted in 4 strong matches. In addition, 5 other profiles were matched to certain referent samples with lower probability.
Our results show that more than 6 decades after the end of the World War II, DNA analysis may significantly contribute to the identification of the remains from that period. Additional analysis of Y-STRs and mitochondrial DNA (mtDNA) markers will be performed in the second phase of the identification project.
介绍三个机构共同努力，鉴定在斯洛文尼亚斯科夫亚洛卡地区两个乱葬坑中发现的二战时期人类遗骸的情况。
在两个位置相近的小型乱葬坑中发现了27具个体的遗骸。使用标准的苯酚/氯仿酒精提取法或优化的Qiagen DNA提取程序从骨骼和牙齿样本中分离DNA。一些回收的样本需要采用额外的DNA纯化方法，如正丁醇处理。使用Quantifiler Human DNA定量试剂盒进行DNA定量。使用PowerPlex 16试剂盒同时扩增15个短串联重复（STR）基因座。使用DNA View程序估计匹配概率。
在所有处理的样本中，15具遗骸在所有15个STR基因座上都获得了完整的图谱。其他12个图谱是部分图谱。最不成功的图谱包括13个基因座。此外，还收集并分析了69份来自潜在在世亲属的参考样本（口腔拭子）。将受害者的图谱与参考样本数据库进行比较，得到了4个强匹配。另外，还有5个图谱与某些参考样本有较低概率的匹配。
我们的结果表明，在二战结束60多年后，DNA分析可能对鉴定那个时期的遗骸有重大贡献。在鉴定项目的第二阶段将进行Y-STR和线粒体DNA（mtDNA）标记的进一步分析。
