Ajaiyeoba E O, Ashidi J S, Okpako L C, Houghton P J, Wright C W
Department of Pharmacognosy, University of Ibadan, Ibadan, Nigeria.
Phytother Res. 2008 Feb;22(2):254-5. doi: 10.1002/ptr.2254.
Cassia siamea L. (Fabaceae) was identified from the southwest Nigerian ethnobotany as a remedy for febrile illness. This led to the bioassay-guided fractionation of stem bark of the plant extract, using the parasite lactate dehydrogenase assay and multi-resistant strain of Plasmodium falciparum (K1) for assessing the in vitro antimalarial activity. Emodin and lupeol were isolated from the ethyl acetate fraction by a combination of chromatographic techniques. The structures of the compounds were determined by spectroscopy, co-spotting with authentic samples and comparison with literature data. Both compounds were found to be the active principles responsible for the antiplasmodial property with IC(50) values of 5 microg/mL, respectively.
腊肠树（豆科）在尼日利亚西南部的民族植物学中被鉴定为治疗发热疾病的药物。这促使对该植物提取物的茎皮进行生物测定导向的分级分离，使用寄生虫乳酸脱氢酶测定法和恶性疟原虫（K1）的多抗性菌株来评估体外抗疟活性。通过色谱技术的组合从乙酸乙酯馏分中分离出大黄素和羽扇豆醇。通过光谱学、与标准样品共点样以及与文献数据比较来确定化合物的结构。发现这两种化合物都是具有抗疟特性的活性成分，其半数抑制浓度（IC50）值分别为5微克/毫升。
