Lee Chia-Lin, Chang Fang-Rong, Hsieh Pei-Wen, Chiang Michael-Y, Wu Chin-Chung, Huang Zih-You, Lan Yu-Hsuan, Chen Mei, Lee Kuo-Hsiung, Yen Hsin-Fu, Hung Wen-Chun, Wu Yang-Chang
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Phytochemistry. 2008 Jan;69(1):276-87. doi: 10.1016/j.phytochem.2007.07.005. Epub 2007 Aug 21.
Seventeen ent-abietane diterpenes, including gelomulides K-X (1-14), and three known compounds, were isolated from a dichloromethane-soluble extract of Gelonium aequoreum through bioassay-guided fractionation. Their structures were identified by spectroscopic methods, and stereochemistry was confirmed by X-ray crystallographic analysis, CD spectral data, and Mosher's method. The isolates were evaluated for in vitro cytotoxic activity, and compounds 1 and 3 showed moderate cytotoxicity against lung (A549), breast (MDA-MB-231 and MCF7), and liver (HepG2) cancer cell lines.
通过生物活性导向分离，从圆叶刺麻的二氯甲烷可溶提取物中分离出17种对映-松香烷二萜，包括格罗木内酯K-X（1-14）以及3种已知化合物。通过光谱方法鉴定其结构，并通过X射线晶体学分析、圆二色光谱数据和莫舍尔方法确定其立体化学。对分离得到的化合物进行了体外细胞毒性活性评估，化合物1和3对肺癌（A549）、乳腺癌（MDA-MB-231和MCF7）和肝癌（HepG2）细胞系显示出中等细胞毒性。
