Hatipoğlu Ahmet, Türkyilmaz Zeliha, Mert Selva
Department of General Surgery, Trakya University, Medical Faculty, 22030 Edirne, Turkey.
Yonsei Med J. 2007 Aug 31;48(4):659-64. doi: 10.3349/ymj.2007.48.4.659.
Postoperative intraabdominal adhesion formation is a major clinical problem. No previous study was found, reporting the relationship between adhesion formation and melatonin administration, but melatonin, a strong antioxidant, is recognized to have certain effects on the progression of adhesion formation mechanism. It was therefore decided to investigate the effects of melatonin on postoperative adhesion formation.
A total number of 24 Sprague-Dawley rats were utilized. Three groups, described as: Group A, sham laparatomy (n=8), Group B, rats that underwent only ischemia-reperfusion (n=8) and Group C, rats that underwent ischemia- reperfusion and were given 10 mg/kg melatonin solution i.v. (n=8). For Groups B and C, the ileocolic vessels were clamped. Blood glutathione peroxidase levels of all study groups were assessed, then microscopic and macroscopic adhesion scores were evaluated.
Glutathione peroxidase levels of the melatonin-treated group were significantly higher and fibroblast proliferation and macroscopic adhesion scores were significantly lower, than in the melatonin-free group.
The results of this study supported the hypothesis, that melatonin administration may prevent intraabdominal adhesions resulting from surgery.
术后腹腔内粘连形成是一个主要的临床问题。此前未发现有研究报道粘连形成与褪黑素给药之间的关系，但褪黑素作为一种强抗氧化剂，被认为对粘连形成机制的进展有一定影响。因此，决定研究褪黑素对术后粘连形成的影响。
总共使用了24只Sprague-Dawley大鼠。分为三组：A组，假手术组（n = 8）；B组，仅接受缺血再灌注的大鼠（n = 8）；C组，接受缺血再灌注并静脉注射10 mg/kg褪黑素溶液的大鼠（n = 8）。对于B组和C组，夹闭回结肠血管。评估所有研究组的血液谷胱甘肽过氧化物酶水平，然后评估微观和宏观粘连评分。
褪黑素治疗组的谷胱甘肽过氧化物酶水平显著高于无褪黑素组，而成纤维细胞增殖和宏观粘连评分显著低于无褪黑素组。
本研究结果支持以下假设，即给予褪黑素可能预防手术引起的腹腔内粘连。
