Tsuboy M S, Angeli J P F, Mantovani M S, Knasmüller S, Umbuzeiro G A, Ribeiro L R
Departamento de Ciências Biológicas, UNESP, Assis, SP, Brazil.
Toxicol In Vitro. 2007 Dec;21(8):1650-5. doi: 10.1016/j.tiv.2007.06.020. Epub 2007 Jul 21.
Textile dyes are discarded into the aquatic ecosystem via industrial effluents and potentially expose humans and local biota to adverse effects. The commercial dye CI Disperse Blue 291 which contains the aminoazobenzene 2-[(2-bromo-4,6-dinitrophenyl)azo]-5(diethylamino)-4-methoxyacetanilide (CAS registry no. 56548-64-2), was tested for genotoxicity and cytotoxicity in the human hepatoma cell line HepG2, using the comet assay, micronucleus (MN) test and a cell viability test. Five different concentrations of the test compound were examined: 200 microg/ml, 400 microg/ml, 600 microg/ml, 800 microg/ml and 1000 microg/ml. An increase in comet tail length and in the frequency of MN was detected with exposure of cells to concentrations of the commercial dye from 400 microg/ml. Furthermore, the dye was found to decrease cell viability. The results of this study demonstrate for the first time the genotoxic and mutagenic effects of the dye CI Disperse Blue 291 in mammalian cells, thus stressing the need to develop non-mutagenic dyes and to invest in improving the treatment of effluents. These measures will help to prevent harmful effects that these compounds can have on humans and aquatic organisms that come in contact with them.
纺织染料通过工业废水被排放到水生生态系统中，可能会使人类和当地生物群受到不良影响。对含有氨基偶氮苯2-[(2-溴-4,6-二硝基苯基)偶氮]-5-(二乙氨基)-4-甲氧基乙酰苯胺（化学物质登记号：56548-64-2）的商用染料CI分散蓝291，采用彗星试验、微核（MN）试验和细胞活力试验，在人肝癌细胞系HepG2中进行了遗传毒性和细胞毒性测试。检测了受试化合物的五种不同浓度：200微克/毫升、400微克/毫升、600微克/毫升、800微克/毫升和1000微克/毫升。当细胞暴露于浓度为400微克/毫升及以上的商用染料时，检测到彗星尾长增加和微核频率增加。此外，发现该染料会降低细胞活力。本研究结果首次证明了染料CI分散蓝291在哺乳动物细胞中的遗传毒性和诱变作用，从而强调了开发无诱变作用染料以及投资改进废水处理的必要性。这些措施将有助于防止这些化合物对接触到它们的人类和水生生物产生有害影响。
