Friedman R M, Costa J R
Infect Immun. 1976 Feb;13(2):487-93. doi: 10.1128/iai.13.2.487-493.1976.
Interferon-treated cultures of Ly cells survived initial infection with high multiplicities of vesicular stomatitis (VSV) or herpes simplex virus (HSV). In the case of HSV, infectious virus and intracellular viral antigen were rapidly eliminated from the interferon-treated cultures, and the cells grew out to form apparently normal monolayers that could be cultured indefinitely. In the VSV-infected Ly cultures, virus titers remained at low levels in interferon-treated cells but after about 14 days rapidly rose and the culture was destroyed. If interferon was added to the medium on days 4 and 6 after infection, virus titers rapidly declined but again recovered and the cells were destroyed. If, however, interferon treatment was resumed 9 days after initial infection, detectable infectious VSV was eliminated from the medium. Several methods, including cocultivation and molecular hybridization, failed to demonstrate persistence of a significant portion of the VSV genome in these cultures.
用干扰素处理过的Ly细胞培养物在最初感染高滴度水疱性口炎病毒（VSV）或单纯疱疹病毒（HSV）后存活下来。就HSV而言，感染性病毒和细胞内病毒抗原在经干扰素处理的培养物中迅速被清除，细胞生长形成明显正常的单层，可无限期培养。在VSV感染的Ly培养物中，在经干扰素处理的细胞中病毒滴度维持在低水平，但约14天后迅速上升，培养物被破坏。如果在感染后第4天和第6天向培养基中添加干扰素，病毒滴度迅速下降，但又恢复，细胞被破坏。然而，如果在初次感染9天后恢复干扰素处理，培养基中可检测到的感染性VSV被清除。包括共培养和分子杂交在内的几种方法未能证明这些培养物中VSV基因组的很大一部分持续存在。
