Ennis H L, Kievitt K D
J Biol Chem. 1976 May 10;251(9):2854-60.
Bacteria that accumulate RNA in the course of inhibition of protein synthesis are impaired in their ability to synthesize beta-galactosidase during subsequent recovery. By contrast, constitutive enzyme synthesis in recovering cells is normal. Even though no beta-galactosidase is made during recovery from this inhibition, a substantial quantity of beta-galactosidase mRNA (as determined by DNA-RNA hybridization) is made. The beta-galactosidase mRNA made in vivo is functional in vitro. It is capable of directing the in vitro synthesis of a portion of the NH2-terminal beta-galactosidase molecule (in the alpha portion of the molecule). However, this protein is not made in vitro. It is concluded that the beta-galactosidase mRNA that is made during recovery from protein synthesis inhibition, although apparently at least partly normally transcribed in vivo and functional in vitro cannot be translated under these conditions in vivo.
在蛋白质合成受抑制过程中积累RNA的细菌，在随后恢复期间合成β-半乳糖苷酶的能力受损。相比之下，恢复中的细胞组成型酶合成正常。尽管从这种抑制中恢复期间不产生β-半乳糖苷酶，但产生了大量的β-半乳糖苷酶mRNA（通过DNA-RNA杂交测定）。体内产生的β-半乳糖苷酶mRNA在体外具有功能。它能够指导体外合成NH2-末端β-半乳糖苷酶分子的一部分（在分子的α部分）。然而，这种蛋白质在体外不产生。得出的结论是，从蛋白质合成抑制中恢复期间产生的β-半乳糖苷酶mRNA，尽管在体内显然至少部分正常转录且在体外具有功能，但在这些体内条件下不能被翻译。
