噬菌体的DNA喷射：关于渗透压抑制实验的一般行为研究
DNA ejection from bacteriophage: towards a general behavior for osmotic-suppression experiments.
作者信息
Castelnovo M, Evilevitch A
机构信息
Laboratoire Joliot-Curie et Laboratoire de Physique, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon cedex 07, France.
出版信息
Eur Phys J E Soft Matter. 2007 Sep;24(1):9-18. doi: 10.1140/epje/i2007-10205-5. Epub 2007 Aug 31.
We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda using the osmotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.
在这项工作中，我们使用渗透抑制技术对从两种不同噬菌体（T5和λ噬菌体）中排出DNA的力进行了体外测量。我们通过重新审视目前关于球形衣壳中DNA包装的理论来分析我们的数据。特别是，我们表明，一个仅基于弯曲考虑的简化分析模型能够定量地解释实验结果。我们还讨论了其物理和生物学意义。
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