酿酒酵母甘油分解代谢缺陷型突变体的分离与鉴定。
Isolation and characterization of Saccharomyces cerevisiae mutants defective in glycerol catabolism.
作者信息
Sprague G F, Cronan J E
出版信息
J Bacteriol. 1977 Mar;129(3):1335-42. doi: 10.1128/jb.129.3.1335-1342.1977.
Abstract
Mutants of the yeast Saccharomyces cerevisiae that are defective in the catabolism of glycerol were isolated, and two types of mutants were obtained. One type was deficient in glycerol kinase activity, whereas the other type was deficient in sn-glycerol 3-phosphate dehydrogenase activity. Genetic analysis indicated that each mutant strain owed its phenotype to a single nuclear mutation, and that the two mutations were complementary. The mutations were not linked to each other or to any of 10 loci tested. In addition, neither mutation was centromere linked. Possible mechanisms for the regulation of these enzymes were tested by growing the parental strain in the presence of various carbon sources.
摘要
分离出了在甘油分解代谢方面存在缺陷的酿酒酵母突变体，并获得了两种类型的突变体。一种类型缺乏甘油激酶活性，而另一种类型缺乏sn-甘油3-磷酸脱氢酶活性。遗传分析表明，每个突变菌株的表型都归因于单个核突变，并且这两个突变是互补的。这些突变彼此之间不连锁，也不与所测试的10个基因座中的任何一个连锁。此外，这两个突变都不与着丝粒连锁。通过在各种碳源存在的情况下培养亲本菌株，测试了这些酶的可能调控机制。
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本文引用的文献
1
Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes.酵母的遗传图谱 IV. 温度敏感基因的图谱绘制以及在定位基因中的二倍体菌株的使用。
Genetics. 1973 May;74(1):33-54. doi: 10.1093/genetics/74.1.33.
2
A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts.对酵母分类中常用的氮同化试验的批判性评价。
J Bacteriol. 1946 Sep;52(3):293-301.
3
ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.大肠杆菌中L-α-甘油磷酸的主动运输
J Biol Chem. 1964 Sep;239:3098-105.
4
Utilization of L-alpha-glycerophosphate by Escherichia coli without hydrolysis.大肠杆菌对L-α-甘油磷酸的非水解利用。
Proc Natl Acad Sci U S A. 1962 Dec 15;48(12):2145-50. doi: 10.1073/pnas.48.12.2145.
5
[Glycerokinase; isolation and properties of the enzyme].[甘油激酶；该酶的分离与性质]
Biochem Z. 1957;329(4):320-31.
6
An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'.一种使用抗生素“制霉菌素”筛选营养缺陷型酵母突变体的富集方法。
Nature. 1966 Jul 9;211(5045):206-7. doi: 10.1038/211206a0.
7
Glycerol metabolism in yeasts. Pathways of utilization and production.酵母中的甘油代谢。利用和产生途径。
Eur J Biochem. 1968 Jul;5(2):165-72. doi: 10.1111/j.1432-1033.1968.tb00353.x.
8
Macromolecule synthesis in temperature-sensitive mutants of yeast.酵母温度敏感突变体中的大分子合成
J Bacteriol. 1967 May;93(5):1662-70. doi: 10.1128/jb.93.5.1662-1670.1967.
9
Purification and properties of glycerol kinase from Escherichia coli.大肠杆菌甘油激酶的纯化及性质
J Biol Chem. 1967 Mar 10;242(5):1030-5.
10
Feedback inhibition of glycerol kinase, a catabolic enzyme in Escherichia coli.甘油激酶的反馈抑制，大肠杆菌中的一种分解代谢酶。
Science. 1966 Aug 12;153(3737):755-7. doi: 10.1126/science.153.3737.755.
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