Hsieh C-H, Lee M-C, Tsai-Wu J-J, Chen M-H, Lee H-S, Chiang H, Herbert Wu C H, Jiang C-C
Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
Osteoarthritis Cartilage. 2008 Mar;16(3):343-51. doi: 10.1016/j.joca.2007.07.001. Epub 2007 Sep 4.
To assess how magnetic fields (MFs), with or without concurrent radio frequency (RF), influence chondrocytes and knee joint repair, we applied an MF strength via magnetic resonance imaging (MRI) slightly greater than the frequently used dosage in the clinics and examined the effects of these treatments in vitro on human chondrocytes and in vivo in pigs.
Human chondrocytes were directly exposed to a 3-tesla (T) magnetic field (MF group) or a 3-T static magnetic field plus 125.3 MHz radio frequency (MF+RF group), and cell proliferation, apoptosis, cytosolic Ca2+ ([Ca2+]i) fluxes and expression of the apoptosis-related proteins of the treated cells were examined to assess the effects of the treatments. In the pig study, we examined the effects of the treatments on the recovery of surgically damaged pig knees.
A 3-T static MF and RF suppressed cell growth and induced apoptosis through p53, p21, p27 and Bax protein expression. In the pig model, we found that MRI surveillance had a deleterious effect on the recovery of the damaged knee cartilage.
Magnetic strength, with or without concurrent RF, suppressed chondrocyte growth in vitro and affected recovery of damaged knee cartilage in vivo in the pig model. These results may be specific to the parameters used in this study and may not apply to other situations, field strengths, forms of cartilage injury, or animal species.
为了评估磁场（MFs）无论有无同时存在射频（RF）时如何影响软骨细胞和膝关节修复，我们通过磁共振成像（MRI）施加了略高于临床常用剂量的MF强度，并在体外研究了这些处理对人软骨细胞的影响，以及在体内对猪的影响。
将人软骨细胞直接暴露于3特斯拉（T）磁场（MF组）或3-T静磁场加125.3 MHz射频（MF+RF组），检测处理后细胞的增殖、凋亡、胞质Ca2+（[Ca2+]i）通量及凋亡相关蛋白表达，以评估处理效果。在猪的研究中，我们检测了这些处理对手术损伤的猪膝关节恢复的影响。
3-T静磁场和射频通过p53、p21、p27和Bax蛋白表达抑制细胞生长并诱导凋亡。在猪模型中，我们发现MRI监测对受损膝关节软骨的恢复有有害影响。
无论有无同时存在RF，磁场强度均在体外抑制软骨细胞生长，并在猪模型中影响体内受损膝关节软骨的恢复。这些结果可能特定于本研究中使用的参数，可能不适用于其他情况、场强、软骨损伤形式或动物物种。
