Kühn I, Allestam G, Stenström T A, Möllby R
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
Appl Environ Microbiol. 1991 Nov;57(11):3171-7. doi: 10.1128/aem.57.11.3171-3177.1991.
A simple, automated microplate system for biochemical characterization of water isolates can be used to obtain fingerprints of the bacterial flora from various water samples. Mathematical models for calculating the diversities and similarities between bacterial populations are described for such fingerprints. The diversity may give information on whether an indigenous or allochthonous flora is present, and the similarities between bacterial populations, as calculated by using a population similarity coefficient (Sp), may indicate contaminations between different water samples. The system was demonstrated on coliform bacterial populations from various water samples, with or without suspected intercontamination. For unrelated water samples, the Sps were close to 0, whereas repeated samples of the same source showed Sps of 0.64 to 0.74. The Sp values from several water samples were also clustered to form a dendrogram, thus indicating the relative similarities between the bacterial populations to confirm suspected common sources of pollution.
一种用于水分离物生化特性分析的简单自动化微孔板系统，可用于获取各种水样中细菌群落的指纹图谱。针对此类指纹图谱，描述了用于计算细菌群体之间多样性和相似性的数学模型。多样性可提供有关本地菌群或外来菌群是否存在的信息，而通过群体相似性系数（Sp）计算得出的细菌群体之间的相似性，可能表明不同水样之间存在污染。该系统在来自各种水样的大肠菌群中进行了演示，这些水样有或没有可疑的交叉污染。对于不相关的水样，Sp值接近0，而同一来源的重复样本显示Sp值在0.64至0.74之间。几个水样的Sp值也被聚类形成树形图，从而表明细菌群体之间的相对相似性，以确认可疑的共同污染源。
