Moore W E, Holdeman L V, Cato E P, Good I J, Smith E P, Ranney R R, Palcanis K G
Infect Immun. 1984 Dec;46(3):720-6. doi: 10.1128/iai.46.3.720-726.1984.
Statistical analyses indicated (i) that the floras of individual samples taken from the depth of sulci with nickel-plated Morse 00 scalers were highly reproducible and representative of the flora present at any given time, (ii) that the different compositions of floras of different people with similar clinical signs were statistically highly significant, and (iii) that floras of different affected sites may differ significantly in some (two of three) people at any one time or may differ from week to week in other people (one of three). Thus the flora composition of individual sites appears to be in dynamic flux, probably in response either to environmental changes or to host responses. There was no evidence that double sampling per se (two single passes with 00 scalers) changed the composition of the flora. Repeat samples taken after 1 week were slightly more similar to the initial samples than were samples taken after 3 weeks.
（i）使用镀镍莫氏00型洁治器从龈沟深度采集的单个样本菌群具有高度可重复性，且能代表任何给定时间的现有菌群；（ii）具有相似临床症状的不同个体的菌群组成差异在统计学上具有高度显著性；（iii）在任何时候，不同患部的菌群在部分人（三分之二）中可能存在显著差异，或者在其他人（三分之一）中可能每周都有所不同。因此，各个部位的菌群组成似乎处于动态变化中，可能是对环境变化或宿主反应的响应。没有证据表明重复采样本身（使用00型洁治器进行两次单次操作）会改变菌群组成。与3周后采集的样本相比，1周后采集的重复样本与初始样本略微更相似。
