Linares Carlos Eduardo Blanco, de Loreto Erico Silva, Silveira Carolina Pereira, Pozzatti Patrícia, Scheid Liliane Alves, Santurio Janio M, Alves Sydney Hartz
Programa de Pós graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Rev Inst Med Trop Sao Paulo. 2007 Jul-Aug;49(4):203-6. doi: 10.1590/s0036-46652007000400001.
Candida dubliniensis is an opportunistic yeast that has been recovered from several body sites in many populations; it is most often recovered from the oral cavities of human immunodeficiency virus-infected patients. Although extensive studies on epidemiology and phylogeny of C. dubliniensis have been performed, little is known about virulence factors such as exoenzymatic and hemolytic activities. In this study we compared proteinase, hyaluronidase, chondroitin sulphatase and hemolytic activities in 18 C. dubliniensis and 30 C. albicans strains isolated from AIDS patients. C. albicans isolates produced higher amounts of proteinase than C. dubliniensis (p < 0.05). All the tested C. dubliniensis strains expressed hyaluronidase and chondroitin sulphatase activities, but none of them were significantly different from those observed with C. albicans (p > 0.05). Hemolytic activity was affected by CaCl2; when this component was absent, we did not notice any significant difference between C. albicans and C. dubliniensis hemolytic activities. On the contrary, when we added 2.5 g% CaCl2, the hemolytic activity was reduced on C. dubliniensis and stimulated on C. albicans tested strains (p < 0.05).
都柏林念珠菌是一种机会性酵母，在许多人群的多个身体部位都有发现；它最常从感染人类免疫缺陷病毒患者的口腔中分离出来。尽管已经对都柏林念珠菌的流行病学和系统发育进行了广泛研究，但对于诸如外酶活性和溶血活性等毒力因子却知之甚少。在本研究中，我们比较了从艾滋病患者中分离出的18株都柏林念珠菌和30株白色念珠菌菌株的蛋白酶、透明质酸酶、硫酸软骨素酶和溶血活性。白色念珠菌分离株产生的蛋白酶量高于都柏林念珠菌（p < 0.05）。所有测试的都柏林念珠菌菌株均表现出透明质酸酶和硫酸软骨素酶活性，但与白色念珠菌观察到的活性均无显著差异（p > 0.05）。溶血活性受氯化钙影响；当不存在该成分时，我们未注意到白色念珠菌和都柏林念珠菌溶血活性之间有任何显著差异。相反，当我们添加2.5 g%氯化钙时，都柏林念珠菌测试菌株的溶血活性降低，而白色念珠菌测试菌株的溶血活性增强（p < 0.05）。
