采用体外试验评估番泻叶（狭叶番泻Cassia angustifolia Vahl.）水提取物的抗诱变和遗传毒性潜力。
Assessment of antimutagenic and genotoxic potential of senna (Cassia angustifolia Vahl.) aqueous extract using in vitro assays.
作者信息
Silva C R, Monteiro M R, Rocha H M, Ribeiro A F, Caldeira-de-Araujo A, Leitão A C, Bezerra R J A C, Pádula M
机构信息
Laboratório de Análise de Toxicidade em Fitoterápicos, Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, UERJ, Rio de Janeiro 20551-030, Brazil.
出版信息
Toxicol In Vitro. 2008 Feb;22(1):212-8. doi: 10.1016/j.tiv.2007.07.008. Epub 2007 Jul 31.
Senna (Cassia angustifolia Vahl.) is widely used as a laxative, although potential side effects, such as toxicity and genotoxicity, have been reported. This study evaluated genotoxic and mutagenic effects of senna aqueous extract (SAE) by means of four experimental assays: inactivation of Escherichia coli cultures; bacterial growth inhibition; reverse mutation test (Mutoxitest) and DNA strand break analysis in plasmid DNA. Our results demonstrated that SAE produces single and double strand breaks in plasmid DNA in a cell free system. On the other hand, SAE was not cytotoxic or mutagenic to Escherichia coli strains tested. In effect, SAE was able to avoid H(2)O(2)-induced mutagenesis and toxicity in Escherichia coli IC203 (uvrA oxyR) and IC205 (uvrA mutM) strains, pointing to a new antioxidant/antimutagenic action of SAE.
番泻叶（狭叶番泻Cassia angustifolia Vahl.）虽有潜在副作用如毒性和遗传毒性的报道，但仍被广泛用作泻药。本研究通过四种实验测定评估了番泻叶水提取物（SAE）的遗传毒性和诱变作用：大肠杆菌培养物的失活；细菌生长抑制；回复突变试验（Mutoxitest）以及质粒DNA中的DNA链断裂分析。我们的结果表明，SAE在无细胞系统中可导致质粒DNA产生单链和双链断裂。另一方面，SAE对所测试的大肠杆菌菌株无细胞毒性或诱变性。实际上，SAE能够避免H(2)O(2)诱导的大肠杆菌IC203（uvrA oxyR）和IC205（uvrA mutM）菌株的诱变和毒性，表明SAE具有新的抗氧化/抗诱变作用。
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