Truco M J, Antonise R, Lavelle D, Ochoa O, Kozik A, Witsenboer H, Fort S B, Jeuken M J W, Kesseli R V, Lindhout P, Michelmore R W, Peleman J
Department of Plant Sciences, University of California, Davis, CA 95616, USA.
Theor Appl Genet. 2007 Oct;115(6):735-46. doi: 10.1007/s00122-007-0599-9. Epub 2007 Sep 8.
An integrated map for lettuce comprising of 2,744 markers was developed from seven intra- and inter-specific mapping populations. A total of 560 markers that segregated in two or more populations were used to align the individual maps. 2,073 AFLP, 152 RFLP, 130 SSR, and 360 RAPD as well as 29 other markers were assigned to nine chromosomal linkage groups that spanned a total of 1,505 cM and ranged from 136 to 238 cM. The maximum interval between markers in the integrated map is 43 cM and the mean interval is 0.7 cM. The majority of markers segregated close to Mendelian expectations in the intra-specific crosses. In the two L. saligna x L. sativa inter-specific crosses, a total of 155 and 116 markers in 13 regions exhibited significant segregation distortion. Data visualization tools were developed to curate, display and query the data. The integrated map provides a framework for mapping ESTs in one core mapping population relative to phenotypes that segregate in other populations. It also provides large numbers of markers for marker assisted selection, candidate gene identification, and studies of genome evolution in the Compositae.
利用七个种内和种间作图群体构建了一张包含2744个标记的生菜整合图谱。共有560个在两个或更多群体中分离的标记用于对各个图谱进行比对。2073个AFLP标记、152个RFLP标记、130个SSR标记、360个RAPD标记以及29个其他标记被定位到9个染色体连锁群上，这些连锁群总长1505厘摩，长度从136厘摩到238厘摩不等。整合图谱中标记间的最大间距为43厘摩，平均间距为0.7厘摩。在种内杂交中，大多数标记的分离情况接近孟德尔预期。在两个柳叶莴苣×栽培莴苣的种间杂交中，13个区域内分别共有155个和116个标记表现出显著的分离畸变。开发了数据可视化工具来管理、展示和查询数据。该整合图谱为在一个核心作图群体中相对于在其他群体中分离的表型对EST进行定位提供了一个框架。它还为标记辅助选择、候选基因鉴定以及菊科基因组进化研究提供了大量标记。
