Syed Naeem H, Sørensen Anker P, Antonise Rudie, van de Wiel Clemens, van der Linden C Gerard, van 't Westende Wendy, Hooftman Danny A P, den Nijs Hans C M, Flavell Andrew J
Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee, DD2 5DA, UK.
Theor Appl Genet. 2006 Feb;112(3):517-27. doi: 10.1007/s00122-005-0155-4. Epub 2005 Dec 10.
Molecular markers based upon a novel lettuce LTR retrotransposon and the nucleotide binding site-leucine-rich repeat (NBS-LRR) family of disease resistance-associated genes have been combined with AFLP markers to generate a 458 locus genetic linkage map for lettuce. A total of 187 retrotransposon-specific SSAP markers, 29 NBS-LRR markers and 242 AFLP markers were mapped in an F(2) population, derived from an interspecific cross between a Lactuca sativa cultivar commonly used in Europe and a wild Lactuca serriola isolate from Northern Europe. The cross has been designed to aid efforts to assess gene flow from cultivated lettuce into the wild in the perspective of genetic modification biosafety. The markers were mapped in nine major and one minor linkage groups spanning 1,266.1 cM, with an average distance of 2.8 cM between adjacent mapped markers. The markers are well distributed throughout the lettuce genome, with limited clustering of different marker types. Seventy-seven of the AFLP markers have been mapped previously and cross-comparison shows that the map from this study corresponds well with the previous linkage map.
基于一种新型生菜LTR反转录转座子以及与抗病相关的核苷酸结合位点-富含亮氨酸重复序列（NBS-LRR）基因家族的分子标记，已与AFLP标记相结合，构建了一个包含458个位点的生菜遗传连锁图谱。总共187个反转录转座子特异性SSAP标记、29个NBS-LRR标记和242个AFLP标记被定位到一个F(2)群体中，该群体源自欧洲常用的栽培生菜品种与来自北欧的野生锯齿生菜分离株之间的种间杂交。此次杂交旨在从转基因生物安全的角度，协助评估栽培生菜向野生环境中的基因流动情况。这些标记被定位到9个主要连锁群和1个次要连锁群中，跨度为1266.1厘摩，相邻定位标记之间的平均距离为2.8厘摩。这些标记在生菜基因组中分布良好，不同标记类型的聚类有限。77个AFLP标记先前已被定位，交叉比较表明本研究构建的图谱与先前的连锁图谱吻合良好。
