Chen Xiaoyi, Liu Liegang, Yao Ping, Yu Dong, Hao Liping, Sun Xiufa
Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2007 Aug;27(4):422-5. doi: 10.1007/s11596-007-0418-1.
In order to study the effect of excessive iodine on immune function of lymphocytes and the role of selenium supplementation with excessive iodine intake, the changes of T lymphocyte number, ratio of subsets, activity of natural killer (NK) cells and lymphocytes proliferation response were investigated. 150 female BALB/C mice were randomly divided into 5 groups in terms of their body weight (n=30 in each group), and 10 of each group were taken as one batch for test. Mice in the 5 groups were orally administrated with iodine 0 (group I), 1500 (group II), 3000 (group III), 6000 microg/L (group IV), iodine 6000 microg/L plus selenium 0.3 mg/L (group V) respectively for 30 days Lymphocyte proliferation response, CD4(+)/CD8(+), Th1/Th2 and the activity of NK cells were measured. CD4(+)/CD8(+) was significantly lower, while lymphocyte proliferation response stronger, and Th1/Th2 and the activity of NK cells significantly higher in group IV than in group I (P<0.01). There was no significant difference in all indexes between group V and group I (P>0.05). It was suggested that excessive iodine as exogenous chemical materials can induce disorders of T lymphocyte immune function in mice. 0.3 mg/L selenium supplementation can protect mice against toxicity induced by 6000 microg/L iodine.
为研究过量碘对淋巴细胞免疫功能的影响以及补硒在过量碘摄入时的作用，观察了T淋巴细胞数量、亚群比例、自然杀伤（NK）细胞活性及淋巴细胞增殖反应的变化。将150只雌性BALB/C小鼠按体重随机分为5组（每组n = 30），每组取10只为一批进行检测。5组小鼠分别经口给予碘0（Ⅰ组）、1500（Ⅱ组）、3000（Ⅲ组）、6000μg/L（Ⅳ组）、碘6000μg/L加硒0.3mg/L（Ⅴ组），连续30天，检测淋巴细胞增殖反应、CD4(+)/CD8(+)、Th1/Th2及NK细胞活性。Ⅳ组CD4(+)/CD8(+)显著低于Ⅰ组，淋巴细胞增殖反应增强，Th1/Th2及NK细胞活性显著高于Ⅰ组（P<0.01）。Ⅴ组与Ⅰ组各项指标比较差异无统计学意义（P>0.05）。提示过量碘作为外源性化学物质可诱导小鼠T淋巴细胞免疫功能紊乱，补充0.3mg/L硒可对抗6000μg/L碘对小鼠的毒性作用。
