Choi Gang-Guk, Bae Myong-Sook, Ahn Chi-Yong, Oh Hee-Mock
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
Biotechnol Lett. 2008 Jan;30(1):87-92. doi: 10.1007/s10529-007-9523-2. Epub 2007 Sep 11.
Arthrospira platensis SAG 21.99 and the isolated bacteria (Halomonas spp., Staphylococcus sp., etc.) from the culture of A. platensis SAG 21.99 were treated with five antibiotics to determine the minimal lethal concentrations. The combination of a washing step and a consecutive treatment with antibiotics, imipenem (100 microg ml(-1)), neomycin (100 microg ml(-1)) and cycloheximide (20 microg ml(-1)), treatment step was highly effective in eliminating bacteria. An axenic culture of A. platensis SAG 21.99 could be induced within 3 days using this method. This technique is a simple and rapid method for obtaining axenic cultures of filamentous cyanobacteria.
将钝顶螺旋藻SAG 21.99以及从钝顶螺旋藻SAG 21.99培养物中分离出的细菌（嗜盐单胞菌属、葡萄球菌属等）用五种抗生素处理，以确定最低致死浓度。洗涤步骤与抗生素连续处理相结合，即亚胺培南（100微克/毫升）、新霉素（100微克/毫升）和放线菌酮（20微克/毫升），该处理步骤在消除细菌方面非常有效。使用此方法可在3天内诱导出钝顶螺旋藻SAG 21.99的无菌培养物。该技术是一种获得丝状蓝细菌无菌培养物的简单快速方法。
