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通过基质辅助激光解吸/电离飞行时间质谱法鉴别野生型和耐氨苄青霉素的大肠杆菌。

Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

作者信息

Camara Johanna E, Hays Faith A

机构信息

Analytical Chemistry Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA.

出版信息

Anal Bioanal Chem. 2007 Nov;389(5):1633-8. doi: 10.1007/s00216-007-1558-7. Epub 2007 Sep 12.

DOI:10.1007/s00216-007-1558-7
PMID:17849103
Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized to discriminate between wild-type and ampicillin-resistant Escherichia coli. Only ampicillin-resistant E. coli displayed an m/z approximately 29,000 peak, which was confirmed as beta-lactamase by in-gel digestion followed by peptide mass fingerprinting. Rapid MALDI-TOF MS detection of antibiotic-resistance could fulfill an important clinical need, providing critical phenotypic information beyond genus-species identification.

摘要

基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）经过优化，用于区分野生型和耐氨苄西林的大肠杆菌。只有耐氨苄西林的大肠杆菌显示出一个质荷比约为29,000的峰，通过凝胶内消化后进行肽质量指纹图谱分析，该峰被确认为β-内酰胺酶。通过MALDI-TOF MS快速检测抗生素耐药性可以满足一项重要的临床需求，提供超出属种鉴定的关键表型信息。

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