Yang J-H, Liu H-X, Zhu G-M, Pan Y-L, Xu L-P, Guo J-H
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.
J Appl Microbiol. 2008 Jan;104(1):91-104. doi: 10.1111/j.1365-2672.2007.03534.x.
To understand the diversity, taxonomy and antagonistic potential of rice-associated bacteria, and to discover new bacteria for biocontrol of rice foliar pathogens.
Amplified ribosomal DNA restriction analysis (ARDRA), BOX-PCR and 16S rRNA gene sequence analysis were used to identify the diversity of 203 rice-associated antagonistic bacteria. Eleven potential biocontrol bacteria were used to test their biological control of rice blast in a natural field experiment. Eleven different genera were encountered in five divisions, including Bacilli, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Deinococci. The most prominent genus in all microenvironments was Bacillus (68 x 5%). The efficacy of rice leaf blast biocontrol was 64 x 35% for strain 1Pe2, 57 x 86% for strain 2R37 and 56 x 44% for strain 1Re14.
Biocontrol data from the field experiments demonstrated no positive correlation between antagonism, physiological characteristics and biocontrol efficacy. There was significant diversity among the rice-associated bacteria isolated from different microenvironments. The most prominent genus of all microenvironments was Bacillus. Brevibacillus brevis strain 1Pe2 and Deinococcus aquaticus strain 1Re14 have good potential for field application and commercial use.
This is the first attempt to study the diversity and identification of rice-associated antagonistic bacteria from different microenvironments, and endophytic bacteria Deinococcus aquaticus strain 1Re14, Acidovorax sp. isolate 3Re21 and Brevibacillus brevis strain 1Pe2 are first reported as rice-associated bacteria.
了解与水稻相关细菌的多样性、分类学及拮抗潜力，发现用于生物防治水稻叶部病原菌的新细菌。
利用核糖体DNA扩增限制性分析（ARDRA）、BOX-PCR及16S rRNA基因序列分析，鉴定203株与水稻相关的拮抗细菌的多样性。在自然田间试验中，用11株潜在的生防细菌测试其对稻瘟病的生物防治效果。在五个分类单元中发现了11个不同的属，包括芽孢杆菌属、α-变形菌纲、β-变形菌纲、γ-变形菌纲和异常球菌纲。在所有微环境中最主要的属是芽孢杆菌属（68×5%）。菌株1Pe2对水稻叶瘟的防治效果为64×35%，菌株2R37为57×86%，菌株1Re14为56×44%。
田间试验的生物防治数据表明，拮抗作用、生理特性与生物防治效果之间无正相关。从不同微环境中分离得到的与水稻相关的细菌存在显著多样性。所有微环境中最主要的属是芽孢杆菌属。短短芽孢杆菌菌株1Pe2和水生栖热菌菌株1Re14具有良好的田间应用和商业应用潜力。
这是首次尝试研究来自不同微环境的与水稻相关的拮抗细菌的多样性和鉴定，内生细菌水生栖热菌菌株1Re14、嗜酸菌属分离株3Re21和短短芽孢杆菌菌株1Pe2首次作为与水稻相关的细菌被报道。
