Turkmen Nihal, Velioglu Y Sedat, Sari Ferda, Polat Gokce
Ankara University, Faculty of Engineering, Department of Food Engineering, 06110-Diskapi-Ankara, Turkey.
Molecules. 2007 Mar 13;12(3):484-96. doi: 10.3390/12030484.
Black tea was extracted for 2, 8 and 18 h with absolute acetone, N,N-dimethyl-formamide (DMF), ethanol and methanol and their 50% aqueous solutions. The extracts were screened for total polyphenol contents, antioxidant and antibacterial activities. The polyphenol content of the extracts was found to be in the range of 0.44-114.01 mg gallic acid equivalents (GAE)/g dry weight tea, depending on the solvent used and the length of the extraction process. In general, aqueous acetone or DMF extracts displayed the highest polyphenol contents and antioxidant activity, while absolute acetone was the least efficient solvent. Antioxidant activities of tea extracts tested using the reducing power and 2,2-diphenyl-1-picryhydrazyl (DPPH) radical methods ranged from 0.09 to 1.18 and from 2.60 to 95.42 %, respectively, depending on the extraction conditions and the antioxidant activities correlated well with the polyphenol concentrations. Aqueous solvent black tea extracts also possessed antibacterial activity, depending on the solvent used and bacterial species tested. Staphylococcus aureus was found to be the most sensitive to all tea extracts, except for the methanol extract. Tea extracts were not effective against Y. enterocolitica, L. monocytogenes and E. coli O157:H7.
红茶分别用无水丙酮、N,N -二甲基甲酰胺(DMF)、乙醇、甲醇及其50%的水溶液提取2小时、8小时和18小时。对提取物进行总多酚含量、抗氧化和抗菌活性的筛选。提取物中的多酚含量在0.44 - 114.01毫克没食子酸当量(GAE)/克干重茶叶范围内,这取决于所使用的溶剂和提取过程的时长。一般来说,丙酮水溶液或DMF提取物的多酚含量和抗氧化活性最高,而无水丙酮是效率最低的溶剂。使用还原力和2,2 -二苯基-1-苦基肼(DPPH)自由基法测试的茶叶提取物的抗氧化活性分别在0.09至1.18以及2.60至95.42%之间,这取决于提取条件,且抗氧化活性与多酚浓度具有良好的相关性。水性溶剂红茶提取物也具有抗菌活性,这取决于所使用的溶剂和测试的细菌种类。发现金黄色葡萄球菌对所有茶叶提取物最敏感,但甲醇提取物除外。茶叶提取物对小肠结肠炎耶尔森菌、单核细胞增生李斯特菌和大肠杆菌O157:H7无效。