Ledwozyw A, Stolarczyk H
Department of Pathophysiology, Veterinary Faculty, Agricultural Academy, Lublin, Poland.
Acta Vet Hung. 1991;39(3-4):175-85.
The influence of free radicals generated by polymorphonuclear leukocytes from bronchopneumonic calves on lipid peroxidation in red blood cells was studied. Incubation of granulocytes with red blood cells causes a rise in malondialdehyde production in these cells in linear dependence on the granulocyte number. Incubation of red blood cells with malondialdehyde causes a generation of adduct moiety of this compound with phosphatidylserine and phosphatidylethanolamine. This adduct is also present in erythrocytes which have been incubated with neutrophils of bronchopneumonic calves. Cytochrome c loaded erythrocyte ghosts showed a high reduction rate of this compound during incubation of ghosts with neutrophils of diseased calves. It is suggested that neutrophils of bronchopneumonic calves are in an activated state in the blood-stream, and free radicals generated by these cells are capable of peroxidizing the red blood cell membrane lipids and causing cross-links between phospholipid moieties in erythrocyte membranes.
研究了支气管肺炎犊牛多形核白细胞产生的自由基对红细胞脂质过氧化的影响。粒细胞与红细胞共孵育会导致这些细胞中丙二醛生成量增加，且与粒细胞数量呈线性相关。红细胞与丙二醛共孵育会导致该化合物与磷脂酰丝氨酸和磷脂酰乙醇胺生成加合物部分。这种加合物也存在于与支气管肺炎犊牛中性粒细胞共孵育的红细胞中。加载细胞色素c的红细胞膜在与患病犊牛中性粒细胞共孵育期间显示出该化合物的高还原率。提示支气管肺炎犊牛的中性粒细胞在血流中处于激活状态，这些细胞产生的自由基能够使红细胞膜脂质过氧化，并导致红细胞膜中磷脂部分之间形成交联。
