在诊断实验室中利用生物学特性对1型和2型人疱疹病毒进行分型。
Use of biological characteristics to type Herpesvirus hominis types 1 and 2 in diagnostic laboratories.
作者信息
Marks-Hellman S, Ho M
出版信息
J Clin Microbiol. 1976 Mar;3(3):277-80. doi: 10.1128/jcm.3.3.277-280.1976.
Abstract
Fifty clinical isolates of Herpesvirus hominis were typed by pock size on chicken embryo chorioallantoic membranes, sensitivity to heparin, and plaque formation on chicken embryo cell cultures. Of 19 isolates that were typed serologically, there was 100% concordance with respect to plaque formation. Pock size was incorrect in one instance and indeterminate in another. Heparin sensitivity was incorrect for one. Of 31 strains that were only biologically characterized, there were four for which heparin sensitivity did not agree with the other two characteristics. Of the three biological tests, the ability to form plaques in chick cell monolayer was the most reliable indicator type.
摘要
用人疱疹病毒的50个临床分离株，通过鸡胚绒毛尿囊膜上的痘疱大小、对肝素的敏感性以及在鸡胚细胞培养物上形成蚀斑的情况进行分型。在19个经血清学分型的分离株中，就蚀斑形成而言，分型结果100%一致。有1个实例的痘疱大小分型错误，另1个实例无法确定。有1个肝素敏感性分型错误。在仅进行生物学特征鉴定的31个菌株中，有4个菌株的肝素敏感性与其他两个特征不一致。在这三种生物学检测方法中，在鸡细胞单层中形成蚀斑的能力是最可靠的分型指标。
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本文引用的文献
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Investigation of plaque formation in chick embryo cells as a biological marker for distinguishing herpes virus type 2 from type 1.以鸡胚细胞中斑块形成作为区分2型疱疹病毒与1型疱疹病毒的生物学标志物的研究。
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Focal necrotic hepatitis in mice as a biological marker for differentiation of Herpesvirus hominis type 1 and type 2.小鼠局灶性坏死性肝炎作为人疱疹病毒1型和2型鉴别的生物学标志物
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Biological markers for differentiation of herpes-virus strains of oral and genital origin.用于区分口腔和生殖器来源疱疹病毒株的生物标志物。
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Proc Soc Exp Biol Med. 1968 Apr;127(4):1022-8. doi: 10.3181/00379727-127-32861.
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