Li Cui-Qin, He Lang-Chong, Dong Hai-Yan, Jin Ju-Qing
School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
J Ethnopharmacol. 2007 Nov 1;114(2):212-7. doi: 10.1016/j.jep.2007.08.002. Epub 2007 Aug 10.
The aim of this study was to screen for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala Koidz. The rhizomes of Atractylodes macrocephala were treated with supercritical CO(2) fluid and the extract was separated by normal-phase and reverse-phase column chromatography. The separated samples were screened with white blood cell membrane (WBCM) chromatography (WBCM-C). The anti-inflammatory effects of these fractions and components were tested pharmacologically in vivo. The results indicated that the retention characteristics of the petrol-ether (1:1, v/v) fraction (BZC-2) of the supercritical CO(2) extract, the atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4, 6-diyn-1-ol isolated from BZC-2 as active fractions and components were similar to that of dexamethasone in WBCM-C. Therefore, they may act on WBCM and its receptors. BZC-2 has shown anti-inflammatory effects in acute and chronic inflammation models in rats and mice. Oral administration of atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-1-ol produced significant anti-inflammatory effects in acute and chronic inflammation models in mice. The screening results with WBCM-C were correlated significantly with pharmacological effects in vivo. Atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-1-ol were the main components of Atractylodes macrocephala that were effective as anti-inflammatory agents.
本研究旨在筛选白术提取物的各组分及化合物的抗炎活性。白术根茎经超临界CO₂流体处理后，提取物通过正相和反相柱色谱进行分离。分离得到的样品采用白细胞细胞膜色谱法（WBCM-C）进行筛选。这些组分和成分的抗炎作用在体内进行了药理学测试。结果表明，超临界CO₂提取物的石油醚（1:1，v/v）馏分（BZC-2）、从BZC-2中分离得到的苍术内酯I和14-乙酰氧基-12-千里光酰氧基十四碳-2E,8E,10E-三烯-4,6-二炔-1-醇作为活性组分，在WBCM-C中的保留特性与地塞米松相似。因此，它们可能作用于白细胞细胞膜及其受体。BZC-2在大鼠和小鼠的急性和慢性炎症模型中均表现出抗炎作用。口服苍术内酯I和14-乙酰氧基-12-千里光酰氧基十四碳-2E,8E,10E-三烯-4,6-二炔-1-醇在小鼠的急性和慢性炎症模型中产生了显著的抗炎作用。WBCM-C的筛选结果与体内药理学效应显著相关。苍术内酯I和14-乙酰氧基-12-千里光酰氧基十四碳-2E,8E,10E-三烯-4,6-二炔-1-醇是白术中作为抗炎剂有效的主要成分。
