Spöler Felix, Frentz Markus, Först Michael, Kurz Heinrich, Schrage Norbert F
Institute of Semiconductor Electronics, RWTH Aachen University, Sommerfeldstrasse 24, 52074 Aachen, Germany.
Burns. 2008 Jun;34(4):549-55. doi: 10.1016/j.burns.2007.05.004. Epub 2007 Sep 14.
So far the study of chemical burns has lacked techniques to define penetration kinetics and the effects of decontamination within biological structures. In this study, we aim to demonstrate that high-resolution optical coherence tomography (HR-OCT) can close this gap. Rabbit corneas were exposed ex vivo to 2.5% hydrofluoric acid (HF) solution, and microstructural changes were monitored in the time domain by OCT imaging. HF application and penetration resulted in shrinkage of the corneal thickness, interpreted as a result of osmolar changes and of loss of water-binding capacity, and a substantial increase in OCT signal amplitudes. The effectiveness of different rinsing solutions on the chemical burn was also evaluated. With tap water and with 1% calcium gluconate, the deep corneal stroma remained clear until the end of the rinsing period but became opaque afterwards. With Hexafluorine, the cornea remained clear for 60 min after rinsing ceased. We conclude that HR-OCT can assist in the clinical evaluation of an ex vivo eye irritation test, and that decontamination of an HF burn using Hexafluorine is efficient.
到目前为止，化学烧伤的研究缺乏定义渗透动力学以及生物结构内去污效果的技术。在本研究中，我们旨在证明高分辨率光学相干断层扫描（HR-OCT）能够填补这一空白。将兔角膜离体暴露于2.5%的氢氟酸（HF）溶液中，并通过OCT成像在时域监测微观结构变化。应用和渗透HF导致角膜厚度收缩，这被解释为渗透压变化和水结合能力丧失的结果，同时OCT信号幅度大幅增加。还评估了不同冲洗液对化学烧伤的效果。用自来水和1%的葡萄糖酸钙冲洗时，直到冲洗期结束深层角膜基质仍保持清晰，但之后变得不透明。用六氟灵冲洗后，角膜在冲洗停止后60分钟内保持清晰。我们得出结论，HR-OCT可辅助离体眼刺激试验的临床评估，并且使用六氟灵对HF烧伤进行去污是有效的。
