Michalek Jaroslav, Svetlikova Petra, Fedora Michal, Klimovic Michal, Klapacova Lenka, Bartosova Drahomira, Hrstkova Hana, Hubacek Jaroslav A
1st Department of Pediatrics, University Hospital Brno, Brno, Czech Republic.
Hum Immunol. 2007 Sep;68(9):756-60. doi: 10.1016/j.humimm.2007.06.003. Epub 2007 Jul 16.
A proinflammatory cytokine interleukin-6 (IL-6) plays an important role in the development, pathogenesis and outcome of SIRS, sepsis and septic shock. We have evaluated the role of the IL-6 gene polymorphisms in pediatric patients. A total of 421 consecutive pediatric patients admitted to the pediatric intensive care unit with fever, systemic inflammatory response syndrome (SIRS), sepsis, severe sepsis, septic shock, or multiple organ distress syndrome (MODS) were studied together with 644 healthy controls. DNA was isolated and two IL-6 gene polymorphisms (G-174>C and G-572>C) were analyzed. The frequencies of both analyzed variants differ significantly between the group of patients and healthy controls (p = 0.02 for G-174>C and p = 0.049 for G-572>C). In addition, genetic analysis of the G-174>C IL-6 gene variant revealed significant differences between the subgroup of febrile patients and subgroup of septic shock (p = 0.0319) and between the subgroup of SIRS and septic shock (p = 0.038). In both cases the negative genotype was CC. No statistically significant differences for the IL-6 gene polymorphism G-572>C were found between the groups of patients with different diagnosis. IL-6 gene polymorphisms G-174>C and G-572>C could be the predictors of risk of development and/or the predictors of the severity of sepsis in children.
促炎细胞因子白细胞介素-6（IL-6）在全身炎症反应综合征（SIRS）、脓毒症和脓毒性休克的发生、发病机制及转归中起重要作用。我们评估了IL-6基因多态性在儿科患者中的作用。共纳入421例连续入住儿科重症监护病房的儿科患者，这些患者伴有发热、全身炎症反应综合征（SIRS）、脓毒症、严重脓毒症、脓毒性休克或多器官功能障碍综合征（MODS），同时纳入644例健康对照。提取DNA并分析两个IL-6基因多态性（G-174>C和G-572>C）。患者组和健康对照组中两个分析变异的频率存在显著差异（G-174>C，p = 0.02；G-572>C，p = 0.049）。此外，对G-174>C IL-6基因变异的基因分析显示，发热患者亚组与脓毒性休克亚组之间（p = 0.0319）以及SIRS亚组与脓毒性休克亚组之间（p = 0.038）存在显著差异。在这两种情况下，阴性基因型均为CC。在不同诊断的患者组之间，未发现IL-6基因多态性G-572>C有统计学显著差异。IL-6基因多态性G-174>C和G-572>C可能是儿童脓毒症发生风险和/或严重程度的预测指标。
