Asaga T, Suzuki K, Umeda M, Sugimasa Y, Takemiya S, Okamoto T
Department of Surgery, Kanagawa Cancer Center Hospital, Japan.
Jpn J Surg. 1991 Nov;21(6):669-75. doi: 10.1007/BF02471053.
In the process of liver regeneration, the participation of various types of growth stimulators and changes in immune responses have been reported. Here, we examined the growth of subcutaneously transplanted AH130 cells and Walker 256 cells after partial hepatectomy. In the case of tumor cells being transplanted on the same day as partial hepatectomy, the increase in tumor size in hepatectomized rats was significantly greater compared with that in non-treated rats or in those having undergone a simple laparotomy. When the transplantation of tumor cells was done on the 7th day after partial hepatectomy, however, the increase was less marked. We also examined the effect of serum obtained from rats after partial hepatectomy on the in vitro growth of these tumor cells. Growth enhancement was observed with medium containing serum drawn from rats 1 to 4 days after partial hepatectomy. These results suggest that the growth of tumor cells was stimulated during liver regeneration and that some humoral factors participated in the process. Furthermore, as the conditions of the in vitro method appear to mimic those of the in vivo method, the in vitro approach should be very useful for analysis of the factors responsible.
在肝脏再生过程中，已有报道各种类型的生长刺激因子参与其中以及免疫反应发生变化。在此，我们研究了部分肝切除术后皮下移植的AH130细胞和Walker 256细胞的生长情况。在与部分肝切除术同一天移植肿瘤细胞的情况下，与未处理的大鼠或仅接受简单剖腹手术的大鼠相比，肝切除大鼠的肿瘤大小增加显著更大。然而，当在部分肝切除术后第7天进行肿瘤细胞移植时，肿瘤大小的增加则不太明显。我们还研究了部分肝切除术后大鼠血清对这些肿瘤细胞体外生长的影响。在含有部分肝切除术后1至4天大鼠血清的培养基中观察到了生长增强。这些结果表明，在肝脏再生过程中肿瘤细胞的生长受到刺激，并且一些体液因子参与了这一过程。此外，由于体外方法的条件似乎模拟了体内方法的条件，因此体外方法对于分析相关因子应该非常有用。
