Hurst A, Hendry G S, Hughes A, Paley B
Can J Microbiol. 1976 May;22(5):677-83. doi: 10.1139/m76-100.
The effect of 45 substances to restore the salt tolerance of sublethally heat-injured Staphylococcus aureus was tested. Sodium pyruvate, yeast extract, L-histine, casitone (Difco), adenosine triphosphate, and acetylphosphate were effective. For enumeration a repair medium was first used, containing sodium pyruvate and penicillin in 1% skim milk. This step was followed by counting on Baird-Parker agar with penicillinase. This method was selective; fewer than 100 staphylococci/g food could be enumerated and it gave counts about 8 times higher than the method of Giolitti and Cantoni used as a five-tube most probable number technique. Heat injury sensitized S. aureus to polymyxin.
测试了45种物质对亚致死热损伤金黄色葡萄球菌恢复耐盐性的影响。丙酮酸钠、酵母提取物、L-组氨酸、酪蛋白胨（Difco）、三磷酸腺苷和乙酰磷酸有效。为了计数，首先使用一种修复培养基，其在1%脱脂乳中含有丙酮酸钠和青霉素。这一步之后是在含有青霉素酶的贝尔德-帕克琼脂上计数。该方法具有选择性；每克食品中少于100个葡萄球菌即可计数，并且其计数结果比用作五管最大可能数技术的焦利蒂和坎托尼方法高约8倍。热损伤使金黄色葡萄球菌对多粘菌素敏感。
