热应激金黄色葡萄球菌中的超氧化物歧化酶活性
Superoxide dismutase activity in thermally stressed Staphylococcus aureus.
作者信息
Bucker E R, Martin S E
出版信息
Appl Environ Microbiol. 1981 Feb;41(2):449-54. doi: 10.1128/aem.41.2.449-454.1981.
Abstract
The effects of heat and NaCl on the activity of superoxide dismutase from Staphylococcus aureus were examined. A linear decrease in superoxide dismutase activity occurred when S. aureus MF-31 cells were thermally stressed for 90 min at 52% C in 100 mM potassium phosphate buffer (pH 7.2). After 20 min of heating, only 5% of the superoxide dismutase activity was lost. Heating for 60, 90 and 120 min resulted in decreases of approximately 10, 22, and 68%, respectively. The rates of thermal inactivation of superoxide dismutase from S. aureus strains 196E and 210 were similar and slightly greater than those of strains MF-31, S-6, and 181. The addition of NaCl before or after heating resulted in increased losses of superoxide dismutase activity.
摘要
研究了热和氯化钠对金黄色葡萄球菌超氧化物歧化酶活性的影响。当金黄色葡萄球菌MF - 31细胞在100 mM磷酸钾缓冲液（pH 7.2）中于52℃热应激90分钟时，超氧化物歧化酶活性呈线性下降。加热20分钟后，仅5%的超氧化物歧化酶活性丧失。加热60、90和120分钟分别导致约10%、22%和68%的活性下降。金黄色葡萄球菌菌株196E和210的超氧化物歧化酶热失活速率相似，略高于菌株MF - 31、S - 6和181。加热前后添加氯化钠会导致超氧化物歧化酶活性损失增加。
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