Moriyama Takahito, Sorokin Andrey
Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Virology. 2008 Feb 20;371(2):336-49. doi: 10.1016/j.virol.2007.09.030. Epub 2007 Oct 31.
Intracellular trafficking of BK Virus (BKV) in human renal proximal tubular epithelial cells (HRPTEC) is critical for BKV nephritis. However, the major trafficking components utilized by BKV remain unknown. Coincubation of HRPTEC with BKV and microtubule disrupting agents prevented BKV infection as detected by immunofluorescence and western blot analysis with antibodies which recognize BKV large T antigen. However, inhibition of a dynein, cellular motor protein, did not interfere with BKV infection in HRPTEC. A colocalization study of BKV with the markers of the endoplasmic reticulum (ER) and the Golgi apparatus (GA), indicated that BKV reached the ER from 6 to 10 h, while bypassing the GA or passing through the GA too transiently to be detected. This study contributes to the understanding of mechanisms of intracellular trafficking used by BKV in the infection of HRPTEC.
BK病毒（BKV）在人肾近端小管上皮细胞（HRPTEC）中的细胞内运输对于BKV肾炎至关重要。然而，BKV利用的主要运输成分仍然未知。通过免疫荧光和使用识别BKV大T抗原的抗体进行的蛋白质印迹分析检测到，HRPTEC与BKV和微管破坏剂共同孵育可预防BKV感染。然而，抑制动力蛋白（一种细胞运动蛋白）并不干扰HRPTEC中的BKV感染。一项BKV与内质网（ER）和高尔基体（GA）标志物的共定位研究表明，BKV在6至10小时到达ER，同时绕过GA或在GA中短暂通过而无法被检测到。这项研究有助于理解BKV在HRPTEC感染中所使用的细胞内运输机制。
