van den Berg Wim B, Joosten Leo A B, van Lent Peter L E M
Radboud University Nijmegen Medical Centre, The Netherlands.
Methods Mol Med. 2007;136:243-53. doi: 10.1007/978-1-59745-402-5_18.
Antigen induced arthritis is a unilateral T-cell driven model caused by direct injection of an antigen into the knee joint of a FCA preimmunized animal. The chronicity is determined by antigen retention in avascular structures of the joint through charge mediated binding or antibody mediated trapping. Cationicity of the antigen is a prerequisite in this model in the mouse and commercial mBSA is a suitable antigen. Cartilage erosive character is strongly enhanced in the presence of marked antibody titer. Concomitant boosting of the immune response with Bordetella pertussis adds to this. T-cell mediated flares can be induced by local or systemic rechallenge with low dose antigen, and display a strong erosive phenotype.
抗原诱导性关节炎是一种由将抗原直接注射到弗氏完全佐剂预免疫动物的膝关节所引发的单侧T细胞驱动模型。其慢性病程由抗原通过电荷介导结合或抗体介导捕获而保留在关节的无血管结构中所决定。在该小鼠模型中，抗原的阳离子性是一个先决条件，商业牛血清白蛋白是一种合适的抗原。在存在显著抗体滴度的情况下，软骨侵蚀特性会显著增强。百日咳博德特氏菌伴随增强免疫反应会加剧这种情况。低剂量抗原的局部或全身再激发可诱导T细胞介导的炎症发作，并呈现出强烈的侵蚀表型。
