Leibold W, Huldt G, Flanagan T D, Andersson M, Dalens M, Wright D H, Voller A, Klein G
Int J Cancer. 1976 Apr 15;17(4):533-41. doi: 10.1002/ijc.2910170417.
Eight squirrel monkeys (Saimiri sciureus) challenged with EBV or EBV-transformed SLCL were naturally or experimentally infected with Plasmodium knowlesi or Pl. brasilianum. Most of the animals had been splenectomized and unilaterally nephrectomized. Three of these monkeys received one dose of 6 to 12 X 10(8) autologous SLCL. These lines were derived from saimiri lymphoid cells permanently transformed by B-EBV in vitro. All three animals developed multiple undifferentiated malignant lymphomas and died 8 to 10 days post inoculation. Necropsy tumor specimens were EBNA-positive and contained 7 to 21 EBV genome equivalents per cell. EBNA- and EA-positive SLCL were established in vitro from four tumor explants of two monkeys. These results demonstrate that in vitro EBV-transformed SLCL are able to cause tumor formation in autologous squirrel monkeys. Five monkeys received high transforming doses of B-EBV or S-EBV, derived from one of the tumorigenic SLCL. None of the animals developed any sign of tumor formation during an observation period of up to 130 days. Three of these monkeys showed no detectable EBV-related seroconversion while the sera of two monkeys had become anti-EBNA-positive when tested at days 28 and 130 respectively post inoculation. Two additional monkeys received neither EBV nor SLCL. They showed no clinical evidence of tumor development or spontaneous seroconversion over a period of more than 1 year.
八只松鼠猴（松鼠猴属）感染了EB病毒或EB病毒转化的SLCL，随后自然感染或实验性感染诺氏疟原虫或巴西疟原虫。大多数动物已接受脾切除术和单侧肾切除术。其中三只猴子接受了一剂6至12×10⁸个自体SLCL。这些细胞系源自体外被B-EBV永久转化的松鼠猴淋巴细胞。所有三只动物均发展为多发性未分化恶性淋巴瘤，并在接种后8至10天死亡。尸检肿瘤标本EBNA呈阳性，每个细胞含有7至21个EB病毒基因组当量。从两只猴子的四个肿瘤外植体中体外建立了EBNA和EA呈阳性的SLCL。这些结果表明，体外EB病毒转化的SLCL能够在自体松鼠猴中导致肿瘤形成。五只猴子接受了来自一种致瘤性SLCL的高转化剂量的B-EBV或S-EBV。在长达130天的观察期内，没有一只动物出现任何肿瘤形成的迹象。其中三只猴子未检测到EB病毒相关的血清转化，而另外两只猴子的血清在接种后第28天和第130天检测时分别变为抗EBNA阳性。另外两只猴子既未接受EB病毒也未接受SLCL。在超过1年的时间里，它们没有肿瘤发展或自发血清转化的临床证据。
