在小核糖核酸病毒培养物中检测1型、3型、4型、5型和6型人细小病毒。
Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures.
作者信息
de Vries Michel, Pyrc Krzysztof, Berkhout Ron, Vermeulen-Oost Wilma, Dijkman Ronald, Jebbink Maarten F, Bruisten Sylvia, Berkhout Ben, van der Hoek Lia
机构信息
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of University of Amsterdam, Amsterdam, The Netherlands.
出版信息
J Clin Microbiol. 2008 Feb;46(2):759-62. doi: 10.1128/JCM.02009-07. Epub 2007 Dec 12.
Abstract
Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.
摘要
对在中和试验中无法分型的微小核糖核酸病毒培养物进行了VP1逆转录聚合酶链反应（RT-PCR）和病毒发现工具（VIDISCA）分析。经常鉴定出人细小病毒（HPeV），其中包括罕见的分离株HPeV-4、HPeV-5和HPeV-6。HPeV-5分离株只能通过VIDISCA扩增，而不能通过VP1 RT-PCR扩增。
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