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本文引用的文献

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Isolation and characterization of novel human parechovirus from clinical samples.从临床样本中分离和鉴定新型人细小病毒
Emerg Infect Dis. 2007 Jun;13(6):889-95. doi: 10.3201/eid1306.060896.
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Fourth human parechovirus serotype.人细小病毒第四血清型。
Emerg Infect Dis. 2006 Oct;12(10):1572-5. doi: 10.3201/eid1210.051647.
3
Analysis of a new human parechovirus allows the definition of parechovirus types and the identification of RNA structural domains.对一种新型人细小病毒的分析有助于确定细小病毒的类型并识别RNA结构域。
J Virol. 2007 Jan;81(2):1013-21. doi: 10.1128/JVI.00584-06. Epub 2006 Sep 27.
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Molecular characterization of a Canadian human parechovirus (HPeV)-3 isolate and its relationship to other HPeVs.一株加拿大人类细小病毒 3 型(HPeV-3)分离株的分子特征及其与其他人类细小病毒的关系。
J Med Virol. 2005 Dec;77(4):566-70. doi: 10.1002/jmv.20493.
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Molecular identification and characterization of two proposed new enterovirus serotypes, EV74 and EV75.两种拟议的新型肠道病毒血清型EV74和EV75的分子鉴定与特性分析
J Gen Virol. 2004 Nov;85(Pt 11):3205-3212. doi: 10.1099/vir.0.80148-0.
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Identification of a new human coronavirus.一种新型人类冠状病毒的鉴定。
Nat Med. 2004 Apr;10(4):368-73. doi: 10.1038/nm1024. Epub 2004 Mar 21.
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Isolation and identification of a novel human parechovirus.一种新型人细小病毒的分离与鉴定。
J Gen Virol. 2004 Feb;85(Pt 2):391-398. doi: 10.1099/vir.0.19456-0.
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Improved molecular identification of enteroviruses by RT-PCR and amplicon sequencing.通过逆转录聚合酶链反应(RT-PCR)和扩增子测序改进肠道病毒的分子鉴定。
J Clin Virol. 2003 Apr;26(3):375-7. doi: 10.1016/s1386-6532(03)00004-0.
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Parechoviruses, a novel group of human picornaviruses.细小病毒,一组新型的人类微小核糖核酸病毒。
Ann Med. 2001 Oct;33(7):466-71. doi: 10.3109/07853890109002095.
10
Arginine-glycine-aspartic acid motif is critical for human parechovirus 1 entry.精氨酸-甘氨酸-天冬氨酸基序对人副肠孤病毒1的进入至关重要。
J Virol. 2001 Oct;75(20):10000-4. doi: 10.1128/JVI.75.20.10000-10004.2001.

在小核糖核酸病毒培养物中检测1型、3型、4型、5型和6型人细小病毒。

Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures.

作者信息

de Vries Michel, Pyrc Krzysztof, Berkhout Ron, Vermeulen-Oost Wilma, Dijkman Ronald, Jebbink Maarten F, Bruisten Sylvia, Berkhout Ben, van der Hoek Lia

机构信息

Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of University of Amsterdam, Amsterdam, The Netherlands.

出版信息

J Clin Microbiol. 2008 Feb;46(2):759-62. doi: 10.1128/JCM.02009-07. Epub 2007 Dec 12.

DOI:10.1128/JCM.02009-07
PMID:18077635
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2238116/
Abstract

Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.

摘要

对在中和试验中无法分型的微小核糖核酸病毒培养物进行了VP1逆转录聚合酶链反应(RT-PCR)和病毒发现工具(VIDISCA)分析。经常鉴定出人细小病毒(HPeV),其中包括罕见的分离株HPeV-4、HPeV-5和HPeV-6。HPeV-5分离株只能通过VIDISCA扩增,而不能通过VP1 RT-PCR扩增。