Nagano Y, Matsuno R, Sasaki Y
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Curr Genet. 1991 Nov;20(5):431-6. doi: 10.1007/BF00317074.
A 5.1 kb segment of pea chloroplast DNA containing the upstream region of petA was sequenced. RNAs produced from this DNA were characterized. This region encodes putative genes for psbK, trnQ, zfpA, psaI, ORF231, and petA. These genes are all on the same reading strand except for psbK. The gene organization is somewhat different from that of tobacco, rice, and liverwort, which lack the psbK-trnQ genes in this region and contain ORF184/185. Northern blot and primer extension analysis show that the pea transcript covers the zfpA-psaI-ORF231-petA gene cluster and trnQ. These results indicated that the psbK-trnQ genes have been rearranged and a new transcription unit was formed.
对一段包含petA上游区域的5.1 kb豌豆叶绿体DNA片段进行了测序。对该DNA产生的RNA进行了表征。该区域编码psbK、trnQ、zfpA、psaI、ORF231和petA的推定基因。除psbK外，这些基因都位于同一阅读链上。该基因组织与烟草、水稻和地钱的有所不同，后三者在该区域缺乏psbK-trnQ基因，而含有ORF184/185。Northern印迹和引物延伸分析表明，豌豆转录本覆盖zfpA-psaI-ORF231-petA基因簇和trnQ。这些结果表明，psbK-trnQ基因发生了重排，并形成了一个新的转录单元。
