Akbulut H, Celik I, Akbulut A
Department of Immunology, Faculty of Medicine, Firat University, 23119, Elazig, Turkey.
Indian J Med Microbiol. 2007 Oct;25(4):387-90. doi: 10.4103/0255-0857.37345.
To determine the serum levels of proinflammatory and some of the Th1/Th2 cytokines in brucellosis and their alterations with treatment and outcome.
Twenty-eight acute and seven subacute brucellosis patients diagnosed clinically were included in the study. Twenty healthy volunteers were also included. Brucella standard tube agglutination tests and blood culture were conducted on all subjects. Cytokine levels of pre- and post-treatment period serum samples were measured by ELISA.
The mean serum levels of IL-6, IFN-gamma and TNF-alpha were significantly higher in brucellosis patients compared to the control group ( P < 0.05). No significant differences were found between patient and control groups in terms of IL-1beta , TGF-beta 1, IL-2, IL-4 and IL-8 levels. There was a positive correlation between IFN-gamma, TNF-alpha and IL-6 levels with CRP levels. IL-6, IFN-gamma and TNF-alpha levels measured after treatment were statistically significantly lower than pre-treatment values ( P < 0.001). No differences were found in the levels of these cytokines between acute and subacute patients' sera. IL-6, IFN-gamma and TNF-alpha levels were higher in acute or subacute brucellosis patients.
Although the levels of the cytokines were decreased significantly with effective and adequate treatment these alterations did not correlate with the extent or activity of the disease.
测定布鲁氏菌病患者血清中促炎细胞因子及部分Th1/Th2细胞因子水平，并观察其在治疗过程及预后中的变化。
本研究纳入28例临床诊断为急性布鲁氏菌病患者和7例亚急性布鲁氏菌病患者，另纳入20名健康志愿者。对所有受试者进行布鲁氏菌标准试管凝集试验和血培养。采用酶联免疫吸附测定法（ELISA）检测治疗前后血清样本中的细胞因子水平。
与对照组相比，布鲁氏菌病患者血清中白细胞介素-6（IL-6）、γ-干扰素（IFN-γ）和肿瘤坏死因子-α（TNF-α）的平均水平显著升高（P<0.05）。患者组与对照组在白细胞介素-1β（IL-1β）、转化生长因子-β1（TGF-β1）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）和白细胞介素-8（IL-8）水平方面未发现显著差异。IFN-γ、TNF-α和IL-6水平与C反应蛋白（CRP）水平呈正相关。治疗后测得的IL-6、IFN-γ和TNF-α水平在统计学上显著低于治疗前值（P<0.001）。急性和亚急性患者血清中这些细胞因子水平未发现差异。急性或亚急性布鲁氏菌病患者的IL-6、IFN-γ和TNF-α水平较高。
尽管有效且充分的治疗使细胞因子水平显著降低，但这些变化与疾病的程度或活动度无关。
