Xie Xiaobo, Jin Fengxue, Song Mi-Hee, Suh Jung-Pil, Hwang Hung-Goo, Kim Yeon-Gyu, McCouch Susan R, Ahn Sang-Nag
College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 305-764, South Korea.
Theor Appl Genet. 2008 Mar;116(5):613-22. doi: 10.1007/s00122-007-0695-x. Epub 2007 Dec 19.
A high-resolution physical map targeting a cluster of yield-related QTLs on the long arm of rice chromosome 9 has been constructed across a 37.4 kb region containing seven predicted genes. Using a series of BC3F4 nearly isogenic lines (NILs) derived from a cross between the Korean japonica cultivar Hwaseongbyeo and Oryza rufipogon (IRGC 105491), a total of seven QTLs for 1,000-grain weight, spikelets per panicle, grains per panicle, panicle length, spikelet density, heading date and plant height were identified in the cluster (P<or=0.0001). All seven QTLs were additive, and alleles from the low-yielding O. rufipogon parent were beneficial in the Hwaseongbyeo background. Yield trials with BC3F4 NILs showed that lines containing a homozygous O. rufipogon introgression in the target region out-yielded sibling NILs containing Hwaseongbyeo DNA by 14.2-17.7%, and out-yielded the Hwaseongbyeo parent by 16.2-23.7%. While higher yielding plants containing the O. rufipogon introgression were also taller and later than controls, the fact that all seven of the QTLs were co-localized in the same 37.4 kb interval suggests the possibility that a single, pleiotropic gene acting as a major regulator of plant development may control this suite of agronomically important plant phenotypes.
已构建了一个高分辨率物理图谱，该图谱针对水稻第9号染色体长臂上与产量相关的QTL簇，跨越一个包含7个预测基因的37.4 kb区域。利用韩国粳稻品种华城稻与野生稻（IRGC 105491）杂交衍生的一系列BC3F4近等基因系（NILs），在该簇中总共鉴定出7个与千粒重、每穗小穗数、每穗粒数、穗长、小穗密度、抽穗期和株高相关的QTL（P≤0.0001）。所有7个QTL均为加性效应，来自低产野生稻亲本的等位基因在华城稻背景下是有益的。对BC3F4 NILs进行的产量试验表明，在目标区域含有纯合野生稻渗入片段的株系比含有华城稻DNA的同胞NILs产量高出14.2 - 17.7%，比华城稻亲本产量高出16.2 - 23.7%。虽然含有野生稻渗入片段的高产植株也比对照更高且抽穗更晚，但所有7个QTL共定位在同一个37.4 kb区间这一事实表明，可能存在一个作为植物发育主要调节因子的单一多效基因控制着这一系列重要的农艺植物表型。
