Droit Arnaud, Hunter Joanna M, Rouleau Michèle, Ethier Chantal, Picard-Cloutier Aude, Bourgais David, Poirier Guy G
Health and Environment Unit, Laval University Medical research Center, CHUQ, Québec, Canada.
BMC Bioinformatics. 2007 Dec 19;8:483. doi: 10.1186/1471-2105-8-483.
In the "post-genome" era, mass spectrometry (MS) has become an important method for the analysis of proteins and the rapid advancement of this technique, in combination with other proteomics methods, results in an increasing amount of proteome data. This data must be archived and analysed using specialized bioinformatics tools.
We herein describe "PARPs database," a data analysis and management pipeline for liquid chromatography tandem mass spectrometry (LC-MS/MS) proteomics. PARPs database is a web-based tool whose features include experiment annotation, protein database searching, protein sequence management, as well as data-mining of the peptides and proteins identified.
Using this pipeline, we have successfully identified several interactions of biological significance between PARP-1 and other proteins, namely RFC-1, 2, 3, 4 and 5.
在“后基因组”时代，质谱分析法（MS）已成为分析蛋白质的重要方法，并且该技术与其他蛋白质组学方法相结合的快速发展，产生了越来越多的蛋白质组数据。这些数据必须使用专门的生物信息学工具进行存档和分析。
我们在此描述“PARPs数据库”，这是一种用于液相色谱串联质谱（LC-MS/MS）蛋白质组学的数据分析和管理流程。PARPs数据库是一个基于网络的工具，其功能包括实验注释、蛋白质数据库搜索、蛋白质序列管理以及对已鉴定的肽段和蛋白质的数据挖掘。
使用这个流程，我们成功鉴定出了PARP-1与其他蛋白质（即RFC-1、2、3、4和5）之间的几种具有生物学意义的相互作用。
