用于沙门氏菌血清分型的商业抗血清评估。
Evaluation of commercial antisera for Salmonella serotyping.
作者信息
Schrader Kimmi N, Fernandez-Castro Ali, Cheung Wendy K W, Crandall Claudia M, Abbott Sharon L
机构信息
Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Public Health, Richmond, California 94804, USA.
出版信息
J Clin Microbiol. 2008 Feb;46(2):685-8. doi: 10.1128/JCM.01808-07. Epub 2007 Dec 19.
We compared a set of commercial Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from the Microbial Diseases Laboratory, California Department of Public Health, using 327 Salmonella enterica strains belonging to subgroups I, II, IIIa, IIIb, and IV. The sensitivities of Denka Seiken (Tokyo, Japan) somatic and flagellar antisera (using a tube agglutination assay) were 94.0% and 99.2%, respectively, and the specificity was 100% for both sets of sera. Polyvalent O and O1 antiserum sensitivity and specificity were >90%, with the exception of polyvalent O1 antiserum, for which sensitivity was 88.9%. When Denka Seiken flagellar antisera were used in a slide agglutination assay, the sensitivity and accuracy dropped to 88.9% and the specificity fell to 91%. Overall, Denka Seiken commercial antisera performed very well and, together with the comprehensive range of factors available, offer laboratories quality reagents suitable for serotyping strains of salmonellae.
我们使用327株属于I、II、IIIa、IIIb和IV亚组的肠炎沙门氏菌菌株，将一组市售的沙门氏菌菌体和鞭毛血清型分型抗血清与加利福尼亚州公共卫生部微生物疾病实验室自行制备的抗血清进行了比较。日本东京Denka Seiken公司的菌体和鞭毛抗血清（采用试管凝集试验）的敏感性分别为94.0%和99.2%，两组血清的特异性均为100%。除了多价O1抗血清敏感性为88.9%外，多价O和O1抗血清的敏感性和特异性均>90%。当使用Denka Seiken鞭毛抗血清进行玻片凝集试验时，敏感性和准确性降至88.9%，特异性降至91%。总体而言，Denka Seiken公司的市售抗血清表现非常出色，并且与现有的一系列综合因素一起，为实验室提供了适用于沙门氏菌菌株血清型分型的优质试剂。
Zotero 插件