在厌氧生长的大肠杆菌K12中，质子转运与电子从内源性底物流向延胡索酸相偶联。
Proton translocation coupled to electron flow from endogenous substrates to fumarate in anaerobically grown Escherichia coli K12.
作者信息
Gutowski S J, Rosenberg H
出版信息
Biochem J. 1977 Apr 15;164(1):265-7. doi: 10.1042/bj1640265.
Abstract
Observed leads to H+/2e- values for proton translocation during the reduction of fumarate by endogenous substrates in anaerobic cells of Escherichia coli K12 varied with fumarate concentration. This variation was probably due mainly to incomplete fumarate utilization. Under optimum conditions a minimum value for leads to H+/2e- of 1.04+/-0.20 was obtained.
摘要
在大肠杆菌K12厌氧细胞中，内源性底物还原富马酸盐过程中观察到的质子转运H⁺/2e⁻值随富马酸盐浓度而变化。这种变化可能主要是由于富马酸盐利用不完全所致。在最佳条件下，获得了H⁺/2e⁻的最小值为1.04±0.20。
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本文引用的文献
1
Respiration-driven proton translocation in rat liver mitochondria.
Biochem J. 1967 Dec;105(3):1147-62. doi: 10.1042/bj1051147.
2
Separation of intermediates of the citric acid cycle and related compounds by thin-layer chromatography.
Anal Biochem. 1966 Nov;17(2):210-3. doi: 10.1016/0003-2697(66)90199-0.
3
The uptake of C4-dicarboxylic acids by Escherichia coli.
Eur J Biochem. 1971 Jan;18(2):274-81. doi: 10.1111/j.1432-1033.1971.tb01240.x.
4
Chemiosmotic interpretation of active transport in bacteria.
Ann N Y Acad Sci. 1974 Feb 18;227:297-311. doi: 10.1111/j.1749-6632.1974.tb14395.x.
5
Translocations through natural membranes.
Adv Enzymol Relat Areas Mol Biol. 1967;29:33-87. doi: 10.1002/9780470122747.ch2.
6
Respiration-driven proton translocation in Escherichia coli.
Biochem J. 1973 Sep;136(1):217-20. doi: 10.1042/bj1360217.
7
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Biochem J. 1976 Dec 15;160(3):813-6. doi: 10.1042/bj1600813.
8
Energy coupling to active transport in anaerobically grown mutants of Escherichia Coli K12.
Biochem J. 1976 Mar 15;154(3):731-4. doi: 10.1042/bj1540731.
9
Anaerobic energy-yielding reaction associated with transhydrogenation from glycerol 3-phosphate to fumarate by an Escherichia coli system.
J Bacteriol. 1975 Dec;124(3):1282-7. doi: 10.1128/jb.124.3.1282-1287.1975.
10
Metabolite transport in mutants of Escherichia coli K12 defective in electron transport and coupled phosphorylation.
Biochem J. 1975 Feb;146(2):417-23. doi: 10.1042/bj1460417.
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