Gandolfi Maria Giovanna, Perut Francesca, Ciapetti Gabriela, Mongiorgi Romano, Prati Carlo
Center of Biomineralogy, Crystallography and Biomaterials, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
J Endod. 2008 Jan;34(1):39-44. doi: 10.1016/j.joen.2007.09.001.
The biocompatibility of innovative tetrasilicate cements proposed for root-end filling restorations was tested. White ProRoot-MTA and AH Plus were used as control. The new cements were mixed with a local anesthetic solution (4% articaine) to form a paste. Human osteoblast-like cells Saos-2 were challenged in short-term cultures (72 hours) with solid materials and with material extracts prepared in culture medium. Cell growth and viability, cellular attachment, and morphologic features were assessed to verify cell/material interactions. No acute toxicity was exerted by the experimental cements in the assay systems. On solid samples Saos-2 adhered and proliferated on all the experimental cements and on MTA. The ultrastructural findings revealed that Saos-2 were able to adhere and to spread. The maintenance of the osteoblastic phenotype on the innovative cements was confirmed by the alkaline phosphatase assay. All experimental cements prepared with articaine supported the growth of bone-like cells, showing suitable properties to be used as canal sealers and root-end filling materials.
对用于根尖充填修复的新型四硅酸盐水泥的生物相容性进行了测试。使用白色ProRoot-MTA和AH Plus作为对照。将新型水泥与局部麻醉溶液（4%阿替卡因）混合制成糊剂。在短期培养（72小时）中，用固体材料和在培养基中制备的材料提取物对人成骨样细胞Saos-2进行刺激。评估细胞生长和活力、细胞附着以及形态学特征，以验证细胞/材料相互作用。在检测系统中，实验性水泥未表现出急性毒性。在固体样品上，Saos-2在所有实验性水泥和MTA上均能黏附并增殖。超微结构研究结果显示，Saos-2能够黏附并铺展。碱性磷酸酶检测证实了新型水泥上成骨细胞表型的维持。所有用阿替卡因制备的实验性水泥都支持类骨细胞的生长，显示出适合用作根管封闭剂和根尖充填材料的特性。
