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使用非分子方法检测呼吸道病毒。

Detection of respiratory viruses using non-molecular based methods.

作者信息

Ginocchio Christine C

机构信息

North Shore-LIJ Health System Laboratories, Department of Laboratory Medicine, Lake Success, NY 11042, USA.

出版信息

J Clin Virol. 2007 Nov;40 Suppl 1:S11-4. doi: 10.1016/S1386-6532(07)70004-5.

DOI:10.1016/S1386-6532(07)70004-5
PMID:18162248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7130009/
Abstract

The detection of respiratory viruses from clinical samples is important for patient management, promoting the rational use of antivirals and antibiotics, implementing infection control measures and for epidemiology studies. Respiratory viruses can be identified using a variety of techniques including direct antigen testing (non-immunofluorescent and immunofluorescent methods), conventional and rapid cell culture. This article presents an overview of each method.

摘要

从临床样本中检测呼吸道病毒对于患者管理、促进抗病毒药物和抗生素的合理使用、实施感染控制措施以及流行病学研究都很重要。呼吸道病毒可以通过多种技术进行鉴定，包括直接抗原检测（非免疫荧光法和免疫荧光法）、传统细胞培养和快速细胞培养。本文概述了每种方法。

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本文引用的文献

1
Role of cell culture for virus detection in the age of technology.细胞培养在技术时代病毒检测中的作用。
Clin Microbiol Rev. 2007 Jan;20(1):49-78. doi: 10.1128/CMR.00002-06.
2
Avian influenza A (H5N1).甲型禽流感（H5N1）
J Clin Virol. 2006 Jan;35(1):2-13. doi: 10.1016/j.jcv.2005.09.002. Epub 2005 Oct 6.
3
Detection of human metapneumovirus in clinical samples by immunofluorescence staining of shell vial centrifugation cultures prepared from three different cell lines.通过对由三种不同细胞系制备的空斑小室离心培养物进行免疫荧光染色，检测临床样本中的人偏肺病毒。
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Discovery of novel human and animal cells infected by the severe acute respiratory syndrome coronavirus by replication-specific multiplex reverse transcription-PCR.通过复制特异性多重逆转录聚合酶链反应发现感染严重急性呼吸综合征冠状病毒的新型人类和动物细胞
J Clin Microbiol. 2004 Jul;42(7):3196-206. doi: 10.1128/JCM.42.7.3196-3206.2004.
5
Rapid and sensitive detection of respiratory virus infections for directed antiviral treatment using R-Mix cultures.使用R-Mix培养物快速灵敏地检测呼吸道病毒感染以进行定向抗病毒治疗。
J Clin Virol. 2002 Feb;24(1-2):107-15. doi: 10.1016/s1386-6532(01)00239-6.
6
R-Mix cells are faster, at least as sensitive and marginally more costly than conventional cell lines for the detection of respiratory viruses.在检测呼吸道病毒方面，R-Mix细胞速度更快，至少与传统细胞系一样敏感，成本略高。
J Clin Virol. 2001 Aug;22(1):101-10. doi: 10.1016/s1386-6532(01)00171-8.
7
Evaluation of R-Mix FreshCells in shell vials for detection of respiratory viruses.用于检测呼吸道病毒的壳瓶中R-Mix新鲜细胞的评估。
J Clin Microbiol. 2000 Dec;38(12):4660-2. doi: 10.1128/JCM.38.12.4660-4662.2000.
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SimulFluor respiratory screen for rapid detection of multiple respiratory viruses in clinical specimens by immunofluorescence staining.SimulFluor呼吸道筛查试剂，用于通过免疫荧光染色快速检测临床标本中的多种呼吸道病毒。
J Clin Microbiol. 2000 Feb;38(2):708-11. doi: 10.1128/JCM.38.2.708-711.2000.
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Impact of sample type on rapid detection of influenza virus A by cytospin-enhanced immunofluorescence and membrane enzyme-linked immunosorbent assay.样本类型对细胞离心涂片增强免疫荧光法和膜酶联免疫吸附测定法快速检测甲型流感病毒的影响。
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Clin Microbiol Rev. 1993 Apr;6(2):150-75. doi: 10.1128/CMR.6.2.150.