用于对喉恶性病变进行体内分类的荧光研究。
Fluorescence investigations to classify malignant laryngeal lesions in vivo.
作者信息
Rydell Roland, Eker Charlotta, Andersson-Engels Stefan, Krogdahl Annelise, Wahlberg Peter, Svanberg Katarina
机构信息
Department of Oto-Rhino-Laryngology, Lund University Hospital, S-221 85 Lund, Sweden.
出版信息
Head Neck. 2008 Apr;30(4):419-26. doi: 10.1002/hed.20719.
BACKGROUND
The extent of surgical resection for malignant laryngeal lesions influences voice quality. An instrument to estimate histopathologic grading of dysplasia in vivo may spare normal tissue without increasing the risk of local failure.
METHODS
Laryngeal lesions (N = 39; 21 after administration of delta-aminolevulinic acid (ALA)) were investigated with laser-induced fluorescence, and the results correlated to histopathologic grading in 4 groups: non/mild dysplasia (I), moderate dysplasia (II), severe dysplasia/cancer in situ (III), and carcinoma.
RESULTS
At 337-nm excitation, there were differences in the fluorescence ratio I(431)/I(390) between groups I/III and carcinoma. Following 405-nm excitation, I(500 nm) group I differed significantly from group III and cancer. The sensitivity for the fluorescence method was 89%, and the specificity was 100%.
CONCLUSIONS
There are differences in the in vivo tissue fluorescence between tissue with different gradings of dysplasia and carcinoma.
背景
喉恶性病变的手术切除范围会影响嗓音质量。一种能够在体内评估发育异常组织病理学分级的仪器，可能会在不增加局部复发风险的情况下保留正常组织。
方法
对喉部病变（N = 39；21例在给予δ-氨基乙酰丙酸（ALA）后）进行激光诱导荧光研究，并将结果与4组的组织病理学分级相关联：非/轻度发育异常（I级）、中度发育异常（II级）、重度发育异常/原位癌（III级）和癌。
结果
在337 nm激发下，I/III级组与癌组之间的荧光比率I(431)/I(390)存在差异。在405 nm激发后，I级组的I(500 nm)与III级组和癌组有显著差异。荧光法的敏感性为89%，特异性为100%。
结论
不同分级的发育异常组织与癌组织在体内组织荧光方面存在差异。
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