Shimoi K, Masuda S, Furugori M, Esaki S, Kinae N
Laboratory of Food Hygiene, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Carcinogenesis. 1994 Nov;15(11):2669-72. doi: 10.1093/carcin/15.11.2669.
The anticlastogenic effect of 12 structurally different flavonoids was investigated in whole body gamma-ray irradiated mice. Each flavonoid was administered to ICR male mice by a single gastric intubation (5 mumol/kg) 6 h before gamma-ray irradiation (1.5 Gy) and the frequency of micronucleated reticulocytes (MNRETs) in peripheral blood was determined. In order to elucidate the mechanism of the anticlastogenic effect of these flavonoids, their antioxidative activities were examined by the thiobarbituric acid method using methyl linoleate and Fenton's reagent (Fe2+/H2O2). Of the 12 flavonoids, luteolin had the most marked effect on reducing the frequencies of MNRETs and also inhibiting lipid peroxidation. However, quercetin tetramethylether, which has methoxy groups instead of hydroxyl groups at the 3,7,3',4'-positions, and phloretin with an open C-ring showed the least anticlastogenic and antioxidative activity. A good correlation (r = 0.717, P < 0.01) was observed between the anticlastogenic activity and the antioxidative activity of the 12 flavonoids. These results suggest that the radioprotective effect of flavonoids in mice may be attributed to the hydroxyl radical scavenging potency in a direct or an endogenous enzyme mediated manner.
研究了12种结构不同的黄酮类化合物对全身γ射线照射小鼠的抗致断裂作用。在γ射线照射（1.5 Gy）前6小时，通过单次胃插管（5 μmol/kg）将每种黄酮类化合物给予ICR雄性小鼠，并测定外周血中微核网织红细胞（MNRETs）的频率。为了阐明这些黄酮类化合物抗致断裂作用的机制，使用亚油酸甲酯和芬顿试剂（Fe2+/H2O2）通过硫代巴比妥酸法检测它们的抗氧化活性。在这12种黄酮类化合物中，木犀草素对降低MNRETs频率和抑制脂质过氧化具有最显著的作用。然而，在3,7,3',4'-位具有甲氧基而非羟基的槲皮素四甲醚以及具有开放C环的根皮素表现出最小的抗致断裂和抗氧化活性。观察到12种黄酮类化合物的抗致断裂活性与抗氧化活性之间具有良好的相关性（r = 0.717，P < 0.01）。这些结果表明，黄酮类化合物对小鼠的辐射防护作用可能归因于以直接或内源性酶介导的方式清除羟基自由基的能力。
